RegulonDB RegulonDB 11.1: Gene Form
   

panC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rpnC panC panB panCp2 panCp2 panCp4 panCp4 TSS_271 TSS_271

Gene      
Name: panC    Texpresso search in the literature
Synonym(s): ECK0132, EG11746, b0133
Genome position(nucleotides): 147944 <-- 148795
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.7
External database links:  
ASAP:
 ABE-0000465
CGSC:
 426
ECHOBASE:
 EB1696
ECOLIHUB:
 panC
OU-MICROARRAY:
 b0133
STRING:
 511145.b0133
COLOMBOS: panC


Product      
Name: pantothenate synthetase
Synonym(s): PanC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 31.598
Isoelectric point: 6.173
Motif(s):
 
Type Positions Sequence Comment
3 -> 278 IIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEAKARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNGTETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIRKMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAGERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLIDNK
30 -> 37 MGNLHDGH UniProt: ATP.
149 -> 152 GEKD UniProt: ATP.
186 -> 189 LSSR UniProt: ATP.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.5 - Coenzyme A and its modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016874 - ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004592 - pantoate-beta-alanine ligase activity
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
biological_process GO:0009058 - biosynthetic process
GO:0015940 - pantothenate biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Birch RM., et al., 2003
[2] Chakrabarti KS., et al., 2006
[3] Rothmann M., et al., 2013
External database links:  
ALPHAFOLD:
 P31663
DIP:
 DIP-10437N
ECOCYC:
 PANTOATE-BETA-ALANINE-LIG-MONOMER
ECOLIWIKI:
 b0133
INTERPRO:
 IPR042176
INTERPRO:
 IPR004821
INTERPRO:
 IPR014729
INTERPRO:
 IPR003721
MINT:
 P31663
MODBASE:
 P31663
PANTHER:
 PTHR21299:SF1
PDB:
 1IHO
PDB:
 3GUZ
PFAM:
 PF02569
PRIDE:
 P31663
PRODB:
 PRO_000023489
REFSEQ:
 NP_414675
SMR:
 P31663
UNIPROT:
 P31663


Operon      
Name: panBC         
Operon arrangement:
Transcription unit        Promoter
panBC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_271 147583 forward nd [RS-EPT-CBR] [4]
  promoter panCp4 148910 reverse nd [COMP-AINF] [5]
  promoter panCp2 148920 reverse nd [COMP-AINF] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Birch RM., O'Byrne C., Booth IR., Cash P., 2003, Enrichment of Escherichia coli proteins by column chromatography on reactive dye columns., Proteomics 3(5):764-76

 [2] Chakrabarti KS., Sarma SP., 2006, NMR assignment of 2H, 13C and 15N labeled amino-terminal domain of apo-pantothenate synthetase from E. coli., J Biomol NMR 36 Suppl 1:38

 [3] Rothmann M., Kang M., Villa R., Ntai I., La Clair JJ., Kelleher NL., Chapman E., Burkart MD., 2013, Metabolic perturbation of an essential pathway: evaluation of a glycine precursor of coenzyme A., J Am Chem Soc 135(16):5962-5

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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