RegulonDB

Gene
Name:	otsA
Synonym(s):	ECK1895, EG11751, b1896
Genome position(nucleotides):	1980188 <-- 1981612
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.19
External database links:

ASAP:	ABE-0006318
CGSC:	18073
ECHOBASE:	EB1701
ECOLIHUB:	otsA
OU-MICROARRAY:	b1896
STRING:	511145.b1896
COLOMBOS:	otsA

Gene

Name:

otsA

Synonym(s):

ECK1895, EG11751, b1896

Genome position(nucleotides):

1980188 <-- 1981612

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

49.19

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

Comment

2 -> 474

SRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPAWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKVATFPKLA

UniProt: Trehalose-6-phosphate synthase.

17 -> 452

HAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPAWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLK

22 -> 23

UniProt: UDP-glucose binding; Sequence Annotation Type: region of interest.

366 -> 370

LVAKE

UniProt: UDP-glucose binding; Sequence Annotation Type: region of interest.

Type

Positions

Sequence

Comment

2 -> 474

UniProt: Trehalose-6-phosphate synthase.

17 -> 452

22 -> 23

UniProt: UDP-glucose binding; Sequence Annotation Type: region of interest.

366 -> 370

LVAKE

UniProt: UDP-glucose binding; Sequence Annotation Type: region of interest.

Multifun Terms (GenProtEC)

1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.9 - misc. glucose metabolism

5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure

5 - cell processes --> 5.5 - adaptations --> 5.5.2 - temperature extremes

Gene Ontology Terms (GO)

cellular_component

GO:0005829 - cytosol

molecular_function

GO:0003824 - catalytic activity
GO:0016757 - glycosyltransferase activity
GO:0016740 - transferase activity
GO:0003825 - alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

biological_process

GO:0006950 - response to stress
GO:0006970 - response to osmotic stress
GO:0006974 - cellular response to DNA damage stimulus
GO:0005992 - trehalose biosynthetic process
GO:0070415 - trehalose metabolism in response to cold stress

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_2235	1982444		reverse	nd	[RS-EPT-CBR]	[11]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_2235

1982444

reverse

[RS-EPT-CBR]

[11]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Guo W., Hao H., Dai M., Wang Y., Huang L., Peng D., Wang X., Wang H., Yao M., Sun Y., Liu Z., Yuan Z., 2012, Development of quinoxaline 1, 4-dioxides resistance in Escherichia coli and molecular change under resistance selection., PLoS One 7(8):e43322
[2] Joseph TC., Rajan LA., Thampuran N., James R., 2010, Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance., Mol Biotechnol 46(1):20-5
[3] Kong X., Liu Y., Gou X., Zhang H., Wang X., Zhang J., 2001, Directed evolution of operon of trehalose-6-phosphate synthase/phosphatase from Escherichia coli., Biochem Biophys Res Commun 280(1):396-400
[4] Li H., Su H., Kim SB., Chang YK., Hong SK., Seo YG., Kim CJ., 2012, Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity., J Biosci Bioeng 113(2):224-32
[5] Nguyen Ado Q., Kim YG., Kim SB., Kim CJ., 2013, Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene., Bioresour Technol 143:531-7
[6] Peng S., Stephan R., Hummerjohann J., Tasara T., 2014, Transcriptional analysis of different stress response genes in Escherichia coli strains subjected to sodium chloride and lactic acid stress., FEMS Microbiol Lett 361(2):131-7
[7] Schultz T., Liu J., Capasso P., de Marco A., 2007, The solubility of recombinant proteins expressed in Escherichia coli is increased by otsA and otsB co-transformation., Biochem Biophys Res Commun 355(1):234-9
[8] Stoebel DM., Dorman CJ., 2010, The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli., Mol Biol Evol 27(9):2105-12
[9] Tondervik A., Torgersen HR., Botnmark HK., Strom AR., 2006, Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli., J Bacteriol 188(12):4218-26
[10] Worden CR., Kovac WK., Dorn LA., Sandrin TR., 2009, Environmental pH affects transcriptional responses to cadmium toxicity in Escherichia coli K-12 (MG1655)., FEMS Microbiol Lett 293(1):58-64
[11] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Guo W., Hao H., Dai M., Wang Y., Huang L., Peng D., Wang X., Wang H., Yao M., Sun Y., Liu Z., Yuan Z., 2012, Development of quinoxaline 1, 4-dioxides resistance in Escherichia coli and molecular change under resistance selection., PLoS One 7(8):e43322

[2] Joseph TC., Rajan LA., Thampuran N., James R., 2010, Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance., Mol Biotechnol 46(1):20-5

[3] Kong X., Liu Y., Gou X., Zhang H., Wang X., Zhang J., 2001, Directed evolution of operon of trehalose-6-phosphate synthase/phosphatase from Escherichia coli., Biochem Biophys Res Commun 280(1):396-400

[4] Li H., Su H., Kim SB., Chang YK., Hong SK., Seo YG., Kim CJ., 2012, Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity., J Biosci Bioeng 113(2):224-32

[5] Nguyen Ado Q., Kim YG., Kim SB., Kim CJ., 2013, Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene., Bioresour Technol 143:531-7

[6] Peng S., Stephan R., Hummerjohann J., Tasara T., 2014, Transcriptional analysis of different stress response genes in Escherichia coli strains subjected to sodium chloride and lactic acid stress., FEMS Microbiol Lett 361(2):131-7

[7] Schultz T., Liu J., Capasso P., de Marco A., 2007, The solubility of recombinant proteins expressed in Escherichia coli is increased by otsA and otsB co-transformation., Biochem Biophys Res Commun 355(1):234-9

[8] Stoebel DM., Dorman CJ., 2010, The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli., Mol Biol Evol 27(9):2105-12

[9] Tondervik A., Torgersen HR., Botnmark HK., Strom AR., 2006, Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli., J Bacteriol 188(12):4218-26

[10] Worden CR., Kovac WK., Dorn LA., Sandrin TR., 2009, Environmental pH affects transcriptional responses to cadmium toxicity in Escherichia coli K-12 (MG1655)., FEMS Microbiol Lett 293(1):58-64

[11] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.