Gene
Name:	otsB
Synonym(s):	ECK1896, EG11752, b1897, otsP
Genome position(nucleotides):	1981587 <-- 1982387
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.56
External database links:

ASAP:	ABE-0006320
CGSC:	18070
ECHOBASE:	EB1702
ECOLIHUB:	otsB
OU-MICROARRAY:	b1897
STRING:	511145.b1897
COLOMBOS:	otsB

Reference(s)
[1] Joseph TC., Rajan LA., Thampuran N., James R., 2010, Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance., Mol Biotechnol 46(1):20-5
[2] Kaasen I., McDougall J., Strom AR., 1994, Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex., Gene 145(1):9-15
[3] Klein W., Ehmann U., Boos W., 1991, The repression of trehalose transport and metabolism in Escherichia coli by high osmolarity is mediated by trehalose-6-phosphate phosphatase., Res Microbiol 142(4):359-71
[4] Kong X., Liu Y., Gou X., Zhang H., Wang X., Zhang J., 2001, Directed evolution of operon of trehalose-6-phosphate synthase/phosphatase from Escherichia coli., Biochem Biophys Res Commun 280(1):396-400
[5] Li H., Su H., Kim SB., Chang YK., Hong SK., Seo YG., Kim CJ., 2012, Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity., J Biosci Bioeng 113(2):224-32
[6] Nguyen Ado Q., Kim YG., Kim SB., Kim CJ., 2013, Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene., Bioresour Technol 143:531-7
[7] Schultz T., Liu J., Capasso P., de Marco A., 2007, The solubility of recombinant proteins expressed in Escherichia coli is increased by otsA and otsB co-transformation., Biochem Biophys Res Commun 355(1):234-9
[8] Seo HS., Koo YJ., Lim JY., Song JT., Kim CH., Kim JK., Lee JS., Choi YD., 2000, Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli., Appl Environ Microbiol 66(6):2484-90
[9] Stoebel DM., Dorman CJ., 2010, The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli., Mol Biol Evol 27(9):2105-12
[10] Tondervik A., Torgersen HR., Botnmark HK., Strom AR., 2006, Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli., J Bacteriol 188(12):4218-26
[11] Woodruff LB., Boyle NR., Gill RT., 2013, Engineering improved ethanol production in Escherichia coli with a genome-wide approach., Metab Eng 17:1-11
[12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.