RegulonDB RegulonDB 11.1: Gene Form
   

purU gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

purU ychJ tyrT Fis Fis Fis ppGpp rssAp rssAp purUp4 purUp4 purUp purUp purUp5 purUp5 purUp3 purUp3 TSS_1557 TSS_1557 TSS_1556 TSS_1556 tyrTp tyrTp

Gene      
Name: purU    Texpresso search in the literature
Synonym(s): ECK1227, EG11819, b1232, tgs, ychI
Genome position(nucleotides): 1287782 <-- 1288624
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 47.21
External database links:  
ASAP:
 ABE-0004138
CGSC:
 35231
ECHOBASE:
 EB1766
ECOLIHUB:
 purU
NCBI-GENE:
 945827
OU-MICROARRAY:
 b1232
STRING:
 511145.b1232
COLOMBOS: purU


Product      
Name: formyltetrahydrofolate deformylase
Synonym(s): PurU, Tgs, YchI
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 31.935
Isoelectric point: 7.0
Motif(s):
 
Type Positions Sequence Comment
8 -> 86 VLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRR UniProt: ACT.
86 -> 262 RIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLTRNEHDQKMADAIDAYQPDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRAL

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.3 - purine ribonucleotide biosynthesis
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.17 - formyl-THF biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016787 - hydrolase activity
GO:0016742 - hydroxymethyl-, formyl- and related transferase activity
GO:0008864 - formyltetrahydrofolate deformylase activity
GO:0042802 - identical protein binding
biological_process GO:0006164 - purine nucleotide biosynthetic process
GO:0006730 - one-carbon metabolic process
GO:0009058 - biosynthetic process
GO:0006189 - 'de novo' IMP biosynthetic process
GO:0034214 - protein hexamerization
External database links:  
ALPHAFOLD:
 P37051
ECOCYC:
 FORMYLTHFDEFORMYL-MONOMER
ECOLIWIKI:
 b1232
INTERPRO:
 IPR041729
INTERPRO:
 IPR044074
INTERPRO:
 IPR036477
INTERPRO:
 IPR004810
INTERPRO:
 IPR002912
INTERPRO:
 IPR002376
MODBASE:
 P37051
PANTHER:
 PTHR42706
PFAM:
 PF00551
PFAM:
 PF01842
PRIDE:
 P37051
PRINTS:
 PR01575
PRODB:
 PRO_000023646
PROSITE:
 PS51671
REFSEQ:
 NP_415748
SMR:
 P37051
UNIPROT:
 P37051


Operon      
Name: ychJ-purU         
Operon arrangement:
Transcription unit        Promoter
purU
ychJ-purU
ychJ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1556 1288063 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1557 1288077 reverse nd [RS-EPT-CBR] [1]
  promoter purUp3 1288751 reverse nd [COMP-AINF], [RS-EPT-CBR] [1], [2]
  promoter purUp5 1288753 reverse nd [COMP-AINF] [2]
  promoter purUp4 1288756 reverse nd [COMP-AINF] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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