RegulonDB RegulonDB 10.9: Gene Form
   

opgG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

opgH opgG opgC yceK terminator anti-terminator anti-anti-terminator TSS_1330 TSS_1330 TSS_1329 (cluster) TSS_1329 (cluster) TSS_1328 TSS_1328 opgGp1 opgGp1 opgGp2 opgGp2

Gene      
Name: opgG    Texpresso search in the literature
Synonym(s): ECK1034, EG11885, b1048, mdoA, mdoG
Genome position(nucleotides): 1109335 --> 1110870 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
49.87
External database links:  
ASAP:
ABE-0003558
CGSC:
31849
ECHOBASE:
EB1831
ECOLIHUB:
mdoG
OU-MICROARRAY:
b1048
STRING:
511145.b1048
COLOMBOS: opgG


Product      
Name: osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
Synonym(s): MdoA, MdoG, OpgG
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 57.912
Isoelectric point: 7.249
Motif(s):
 
Type Positions Sequence
23 -> 511 FSIDDVAKQAQSLAGKGYETPKSNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATAVKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVIGAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAYKFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNGLSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPSAWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDEDKLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTSIGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLPANE
1 -> 22 MMKMRWLSAAVMLTLYTSSSWA

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.6 - osmoregulated periplasmic glucan
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
molecular_function GO:0003824 - catalytic activity
GO:0030246 - carbohydrate binding
biological_process GO:0016051 - carbohydrate biosynthetic process
GO:0005975 - carbohydrate metabolic process
GO:0006970 - response to osmotic stress
GO:0009250 - glucan biosynthetic process
GO:0051274 - beta-glucan biosynthetic process
Note(s): Note(s): ...[more].
Evidence: [IMP] Inferred from mutant phenotype
Reference(s): [1] Geiger O., et al., 1992
[2] Hanoulle X., et al., 2004
[3] Lacroix JM., et al., 1991
[4] Schneider JE., et al., 1979
[5] Schulman H., et al., 1977
[6] Schulman H., et al., 1977
[7] Weissborn AC., et al., 1984
External database links:  
DIP:
DIP-10177N
ECOCYC:
EG11885-MONOMER
ECOLIWIKI:
b1048
INTERPRO:
IPR013783
INTERPRO:
IPR023704
INTERPRO:
IPR014756
INTERPRO:
IPR014718
INTERPRO:
IPR007444
INTERPRO:
IPR011013
INTERPRO:
IPR014438
PANTHER:
PTHR30504
PDB:
1TXK
PFAM:
PF04349
PRIDE:
P33136
PRODB:
PRO_000023180
REFSEQ:
NP_415566
SMR:
P33136
UNIPROT:
P33136


Operon      
Name: opgGH         
Operon arrangement:
Transcription unit        Promoter
mdoGH
mdoGH
mdoGH


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1328 1110128 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1329 (cluster) 1110416 forward For this promoter, there
Read more >
[RS-EPT-CBR] [8]
  promoter TSS_1330 1111377 forward nd [RS-EPT-CBR] [8]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Geiger O., Russo FD., Silhavy TJ., Kennedy EP., 1992, Membrane-derived oligosaccharides affect porin osmoregulation only in media of low ionic strength., J Bacteriol 174(4):1410-3

 [2] Hanoulle X., Rollet E., Clantin B., Landrieu I., Odberg-Ferragut C., Lippens G., Bohin JP., Villeret V., 2004, Structural analysis of Escherichia coli OpgG, a protein required for the biosynthesis of osmoregulated periplasmic glucans., J Mol Biol 342(1):195-205

 [3] Lacroix JM., Loubens I., Tempete M., Menichi B., Bohin JP., 1991, The mdoA locus of Escherichia coli consists of an operon under osmotic control., Mol Microbiol 5(7):1745-53

 [4] Schneider JE., Reinhold V., Rumley MK., Kennedy EP., 1979, Structural studies of the membrane-derived oligosaccharides of Escherichia coli., J Biol Chem 254(20):10135-8

 [5] Schulman H., Kennedy EP., 1977, Relation of turnover of membrane phospholipids to synthesis of membrane-derived oligosaccharides of Escherichia coli., J Biol Chem 252(12):4250-5

 [6] Schulman H., Kennedy EP., 1977, Identification of UDP-glucose as an intermediate in the biosynthesis of the membrane-derived oligosaccharides of Escherichia coli., J Biol Chem 252(18):6299-303

 [7] Weissborn AC., Kennedy EP., 1984, Biosynthesis of membrane-derived oligosaccharides. Novel glucosyltransferase system from Escherichia coli for the elongation of beta 1----2-linked polyglucose chains., J Biol Chem 259(20):12644-51

 [8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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