RegulonDB RegulonDB 10.10: Gene Form
   

opgH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

opgH opgG opgC yceK terminator anti-terminator anti-anti-terminator TSS_1330 TSS_1330 TSS_1329 (cluster) TSS_1329 (cluster) TSS_1328 TSS_1328 opgGp1 opgGp1 opgGp2 opgGp2

Gene      
Name: opgH    Texpresso search in the literature
Synonym(s): ECK1035, EG11886, b1049, mdoA, mdoH
Genome position(nucleotides): 1110863 --> 1113406 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.95
External database links:  
ASAP:
ABE-0003560
CGSC:
31852
ECHOBASE:
EB1832
ECOLIHUB:
mdoH
OU-MICROARRAY:
b1049
STRING:
511145.b1049
COLOMBOS: opgH


Product      
Name: osmoregulated periplasmic glucans biosynthesis protein H
Synonym(s): MdoA, MdoH, OPG biosynthesis protein OpgH, OPG glycosyltransferase, OpgH
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 96.937
Isoelectric point: 8.843
Motif(s):
 
Type Positions Sequence
194 -> 214 ILILFAVLFCWVSAGFWTALM
603 -> 625 VTLSLLLEVLFSVLLAPVRMLFH
680 -> 700 FLFWLAPIVFSLILSPFVSVI
19 -> 19 A
289 -> 289 P

 

Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0016757 - glycosyltransferase activity
GO:0016758 - hexosyltransferase activity
GO:0016740 - transferase activity
biological_process GO:0009250 - glucan biosynthetic process
GO:0008361 - regulation of cell size
GO:1900727 - osmoregulated periplasmic glucan biosynthetic process
Note(s): Note(s): ...[more].
Evidence: [AUP] Assay of unpurified protein
[IMP] Inferred from mutant phenotype
Reference(s): [1] Bohin JP., et al., 1984
[2] Bohringer J., et al., 1995
[3] Bontemps-Gallo S., et al., 2017
[4] Bontemps-Gallo S., et al., 2013
[5] Ebel W., et al., 1997
[6] Hill NS., et al., 2013
[7] Lacroix JM., et al., 2010
[8] Lacroix JM., et al., 1991
[9] Lacroix JM., et al., 1989
[10] Loubens I., et al., 1993
[11] Miller KJ., et al., 1986
[12] Murakami K., et al., 2021
[13] Schneider JE., et al., 1979
[14] Schulman H., et al., 1977
[15] Schulman H., et al., 1977
[16] Tang L., et al., 1997
[17] Weissborn AC., et al., 1984
[18] Weissborn AC., et al., 1991
External database links:  
ECOCYC:
EG11886-MONOMER
ECOLIWIKI:
b1049
INTERPRO:
IPR023725
INTERPRO:
IPR029044
INTERPRO:
IPR001173
MODBASE:
P62517
PFAM:
PF00535
PRIDE:
P62517
PRODB:
PRO_000023181
REFSEQ:
NP_415567
UNIPROT:
P62517


Operon      
Name: opgGH         
Operon arrangement:
Transcription unit        Promoter
mdoGH
mdoGH
mdoGH


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1328 1110128 forward nd [RS-EPT-CBR] [19]
  promoter TSS_1329 (cluster) 1110416 forward nd [RS-EPT-CBR] [19]
  promoter TSS_1330 1111377 forward nd [RS-EPT-CBR] [19]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Bohin JP., Kennedy EP., 1984, Regulation of the synthesis of membrane-derived oligosaccharides in Escherichia coli. Assay of phosphoglycerol transferase I in vivo., J Biol Chem 259(13):8388-93

 [2] Bohringer J., Fischer D., Mosler G., Hengge-Aronis R., 1995, UDP-glucose is a potential intracellular signal molecule in the control of expression of sigma S and sigma S-dependent genes in Escherichia coli., J Bacteriol 177(2):413-22

 [3] Bontemps-Gallo S., Bohin JP., Lacroix JM., 2017, Osmoregulated Periplasmic Glucans., EcoSal Plus 7(2)

 [4] Bontemps-Gallo S., Cogez V., Robbe-Masselot C., Quintard K., Dondeyne J., Madec E., Lacroix JM., 2013, Biosynthesis of osmoregulated periplasmic glucans in Escherichia coli: the phosphoethanolamine transferase is encoded by opgE., Biomed Res Int 371429

 [5] Ebel W., Vaughn GJ., Peters HK., Trempy JE., 1997, Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli., J Bacteriol 179(21):6858-61

 [6] Hill NS., Buske PJ., Shi Y., Levin PA., 2013, A moonlighting enzyme links Escherichia coli cell size with central metabolism., PLoS Genet 9(7):e1003663

 [7] Lacroix JM., Bohin JP., 2010, Osmoregulated periplasmic glucan polymerization requires constant protein synthesis in Escherichia coli., Curr Microbiol 61(5):396-400

 [8] Lacroix JM., Loubens I., Tempete M., Menichi B., Bohin JP., 1991, The mdoA locus of Escherichia coli consists of an operon under osmotic control., Mol Microbiol 5(7):1745-53

 [9] Lacroix JM., Tempete M., Menichi B., Bohin JP., 1989, Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli., Mol Microbiol 3(9):1173-82

 [10] Loubens I., Debarbieux L., Bohin A., Lacroix JM., Bohin JP., 1993, Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae., Mol Microbiol 10(2):329-40

 [11] Miller KJ., Kennedy EP., Reinhold VN., 1986, Osmotic adaptation by gram-negative bacteria: possible role for periplasmic oligosaccharides., Science 231(4733):48-51

 [12] Murakami K., Nasu H., Fujiwara T., Takatsu N., Yoshida N., Furuta K., Kaito C., 2021, Absence of osmoregulated periplasmic glucan confers antimicrobial resistance and increases virulence in Escherichia coli., J Bacteriol

 [13] Schneider JE., Reinhold V., Rumley MK., Kennedy EP., 1979, Structural studies of the membrane-derived oligosaccharides of Escherichia coli., J Biol Chem 254(20):10135-8

 [14] Schulman H., Kennedy EP., 1977, Relation of turnover of membrane phospholipids to synthesis of membrane-derived oligosaccharides of Escherichia coli., J Biol Chem 252(12):4250-5

 [15] Schulman H., Kennedy EP., 1977, Identification of UDP-glucose as an intermediate in the biosynthesis of the membrane-derived oligosaccharides of Escherichia coli., J Biol Chem 252(18):6299-303

 [16] Tang L., Weissborn AC., Kennedy EP., 1997, Domains of Escherichia coli acyl carrier protein important for membrane-derived-oligosaccharide biosynthesis., J Bacteriol 179(11):3697-705

 [17] Weissborn AC., Kennedy EP., 1984, Biosynthesis of membrane-derived oligosaccharides. Novel glucosyltransferase system from Escherichia coli for the elongation of beta 1----2-linked polyglucose chains., J Biol Chem 259(20):12644-51

 [18] Weissborn AC., Rumley MK., Kennedy EP., 1991, Biosynthesis of membrane-derived oligosaccharides. Membrane-bound glucosyltransferase system from Escherichia coli requires polyprenyl phosphate., J Biol Chem 266(13):8062-7

 [19] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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