RegulonDB RegulonDB 11.1: Gene Form
   

dusA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dusA yjbM pspG IHF PspF terminator anti-terminator anti-anti-terminator pspGp pspGp dusAp6 dusAp6 dusAp9 dusAp9 TSS_4886 TSS_4886 TSS_4885 TSS_4885

Gene      
Name: dusA    Texpresso search in the literature
Synonym(s): ECK4041, EG11932, b4049, yjbN
Genome position(nucleotides): 4261669 --> 4262706
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.66
External database links:  
ASAP:
 ABE-0013258
ECHOBASE:
 EB1876
ECOLIHUB:
 dusA
OU-MICROARRAY:
 b4049
STRING:
 511145.b4049
COLOMBOS: dusA


Product      
Name: tRNA-dihydrouridine synthase A
Synonym(s): DusA, YjbN
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 38.468
Isoelectric point: 6.576
Motif(s):
 
Type Positions Sequence Comment
30 -> 339 VAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALK
32 -> 34 PML UniProt: FMN.
114 -> 114 C UniProt: Loss of enzymatic activity; when associated with Ala-153..
153 -> 153 K UniProt: Loss of the ability to bind FMN. Loss of enzymatic activity; when associated with Ala-114..
226 -> 228 NGG UniProt: FMN.

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016491 - oxidoreductase activity
GO:0003723 - RNA binding
GO:0000049 - tRNA binding
GO:0050660 - flavin adenine dinucleotide binding
GO:0010181 - FMN binding
GO:0017150 - tRNA dihydrouridine synthase activity
GO:0102264 - tRNA-dihydrouridine20 synthase activity
biological_process GO:0008033 - tRNA processing
GO:0002943 - tRNA dihydrouridine synthesis
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P32695
ECOCYC:
 EG11932-MONOMER
ECOLIWIKI:
 b4049
INTERPRO:
 IPR035587
INTERPRO:
 IPR018517
INTERPRO:
 IPR013785
INTERPRO:
 IPR001269
INTERPRO:
 IPR004653
MODBASE:
 P32695
PANTHER:
 PTHR42907
PFAM:
 PF01207
PRIDE:
 P32695
PRODB:
 PRO_000022490
PROSITE:
 PS01136
REFSEQ:
 NP_418473
SMR:
 P32695
UNIPROT:
 P32695


Operon      
Name: dusA         
Operon arrangement:
Transcription unit        Promoter
dusA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4885 4261470 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4886 4261594 forward nd [RS-EPT-CBR] [1]
  promoter dusAp9 4261600 forward nd [COMP-AINF] [2]
  promoter dusAp6 4261658 forward nd [COMP-AINF] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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