RegulonDB RegulonDB 11.1: Gene Form
   

psuG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fruA psuT psuK psuG TSS_2462 TSS_2462 psuGp4 psuGp4 psuTp2 psuTp2

Gene      
Name: psuG    Texpresso search in the literature
Synonym(s): ECK2158, EG12033, b2165, pscG, yeiN
Genome position(nucleotides): 2257429 <-- 2258367
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.73
External database links:  
ASAP:
 ABE-0007165
ECHOBASE:
 EB1968
ECOLIHUB:
 pscG
OU-MICROARRAY:
 b2165
STRING:
 511145.b2165
COLOMBOS: psuG


Product      
Name: pseudouridine-5'-phosphate glycosidase
Synonym(s): PscG, PsuG, YeiN
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 32.91
Isoelectric point: 5.194
Motif(s):
 
Type Positions Sequence Comment
16 -> 306 EVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMVVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKE
31 -> 31 E UniProt: 7500-fold decrease in reaction rate while little change in substrate affinity..
93 -> 93 K UniProt: 17-fold decrease in reaction rate while modest decrease in substrate affinity..
147 -> 149 SAD UniProt: Substrate binding; Sequence Annotation Type: region of interest.
149 -> 149 D UniProt: Loss of activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules
Gene Ontology Terms (GO)  
cellular_component GO:0032991 - protein-containing complex
molecular_function GO:0016787 - hydrolase activity
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0016798 - hydrolase activity, acting on glycosyl bonds
GO:0004730 - pseudouridylate synthase activity
GO:0030145 - manganese ion binding
GO:0042802 - identical protein binding
biological_process GO:0008152 - metabolic process
GO:0046113 - nucleobase catabolic process
GO:0001522 - pseudouridine synthesis
External database links:  
ALPHAFOLD:
 P33025
DIP:
 DIP-11926N
ECOCYC:
 EG12033-MONOMER
ECOLIWIKI:
 b2165
INTERPRO:
 IPR022830
INTERPRO:
 IPR007342
PANTHER:
 PTHR42909
PDB:
 4GIM
PDB:
 4GIL
PDB:
 4GIJ
PDB:
 4GIK
PFAM:
 PF04227
PRIDE:
 P33025
PRODB:
 PRO_000023625
REFSEQ:
 NP_416670
SMR:
 P33025
SWISSMODEL:
 P33025
UNIPROT:
 P33025


Operon      
Name: psuKG         
Operon arrangement:
Transcription unit        Promoter
psuKG


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter psuTp2 2257496 reverse nd [COMP-AINF] [1]
  promoter psuGp4 2258557 reverse nd [COMP-AINF] [1]
  promoter TSS_2462 2260442 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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