RegulonDB RegulonDB 10.9: Gene Form
   

psuG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fruA psuT psuK psuG TSS_2462 TSS_2462 psuGp4 psuGp4 psuTp2 psuTp2

Gene      
Name: psuG    Texpresso search in the literature
Synonym(s): ECK2158, EG12033, b2165, pscG, yeiN
Genome position(nucleotides): 2257429 <-- 2258367 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.73
External database links:  
ASAP:
 ABE-0007165
ECHOBASE:
 EB1968
ECOLIHUB:
 pscG
OU-MICROARRAY:
 b2165
STRING:
 511145.b2165
COLOMBOS: psuG


Product      
Name: pseudouridine-5'-phosphate glycosidase
Synonym(s): PscG, PsuG, YeiN
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 32.91
Isoelectric point: 5.194
Motif(s):
 
Type Positions Sequence
147 -> 149 SAD
166 -> 166 K
289 -> 289 N
16 -> 306 EVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMVVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKE
31 -> 31 E

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules
Gene Ontology Terms (GO)  
cellular_component GO:0032991 - protein-containing complex
molecular_function GO:0016787 - hydrolase activity
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0016798 - hydrolase activity, acting on glycosyl bonds
GO:0004730 - pseudouridylate synthase activity
GO:0030145 - manganese ion binding
GO:0042802 - identical protein binding
biological_process GO:0008152 - metabolic process
GO:0046113 - nucleobase catabolic process
GO:0001522 - pseudouridine synthesis
External database links:  
DIP:
 DIP-11926N
ECOCYC:
 EG12033-MONOMER
ECOLIWIKI:
 b2165
INTERPRO:
 IPR007342
INTERPRO:
 IPR022830
PANTHER:
 PTHR42909
PDB:
 4GIM
PDB:
 4GIL
PDB:
 4GIK
PDB:
 4GIJ
PFAM:
 PF04227
PRIDE:
 P33025
PRODB:
 PRO_000023625
REFSEQ:
 NP_416670
SMR:
 P33025
SWISSMODEL:
 P33025
UNIPROT:
 P33025


Operon      
Name: psuKG         
Operon arrangement:
Transcription unit        Promoter
psuKG


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter psuTp2 2257496 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter psuGp4 2258557 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter TSS_2462 2260442 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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