RegulonDB

RegulonDB RegulonDB 10.9: Gene Form

yeiE gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	yeiE
Synonym(s):	ECK2150, EG12105, b2157
Genome position(nucleotides):	2248737 <-- 2249618 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.74
External database links:

ASAP:	ABE-0007138
ECHOBASE:	EB2029
ECOLIHUB:	yeiE
OU-MICROARRAY:	b2157
STRING:	511145.b2157
COLOMBOS:	yeiE

Product

Name:

LysR-type transcriptional regulator YeiE

Synonym(s):

YeiE

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

32.724

Isoelectric point:

6.517

Motif(s):

Type	Positions	Sequence
	86 -> 286	EDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYC
	1 -> 60	MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVN
	5 -> 64	LRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGR
	20 -> 39	TTQASVMLALSQSAVSAALT

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0003700 - DNA-binding transcription factor activity GO:0000976 - transcription regulatory region sequence-specific DNA binding
	biological_process	GO:0006351 - transcription, DNA-templated GO:0006355 - regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[HIFS] Human inference of function from sequence
[IMP] Inferred from mutant phenotype
[IPI] Inferred from physical interaction

Reference(s):

[1] Fujii T., et al., 2002
[2] Gao Y., et al., 2018
[3] Perez-Rueda E., et al., 2000
[4] Perez-Rueda E., et al., 2004

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PRIDE:

PRINTS:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

yeiE

Operon arrangement:

Transcription unit	Promoter
yeiE

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2459	2249471	reverse	nd	[RS-EPT-CBR]	[5]
promoter	yeiEp7	2249638	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.994. P-value: 2.902e-6 Read more >	[ICWHO]	[6]
promoter	TSS_2460	2249642	reverse	nd	[RS-EPT-CBR]	[5]
promoter	yeiEp6	2249656	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.573. P-value: 7.192e-5 Read more >	[ICWHO]	[6]
promoter	yeiHp7	2249666	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.343. P-value: 1.542e-5 Read more >	[ICWHO]	[6]
promoter	TSS_2461	2249700	forward	nd	[RS-EPT-CBR]	[5]
promoter	yeiEp5	2249711	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 3.285. P-value: 6.507e-5 Read more >	[ICWHO]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Fujii T., Aritoku Y., Agematu H., Tsunekawa H., 2002, Increase in the rate of L-pipecolic acid production using lat-expressing Escherichia coli by lysP and yeiE amplification., Biosci Biotechnol Biochem 66(9):1981-4
[2] Gao Y., Yurkovich JT., Seo SW., Kabimoldayev I., Drager A., Chen K., Sastry AV., Fang X., Mih N., Yang L., Eichner J., Cho BK., Kim D., Palsson BO., 2018, Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655., Nucleic Acids Res 46(20):10682-10696
[3] Perez-Rueda E., Collado-Vides J., 2000, The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12., Nucleic Acids Res 28(8):1838-47
[4] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RegulonDB