RegulonDB RegulonDB 11.1: Gene Form
   

mukF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mukB mukF cmoM elyC mukE ycbJ CpxR TSS_1197 TSS_1197 TSS_1196 TSS_1196 TSS_1195 TSS_1195 TSS_1194 TSS_1194 cmoMp cmoMp

Gene      
Name: mukF    Texpresso search in the literature
Synonym(s): ECK0913, EG12165, b0922, kicB
Genome position(nucleotides): 974319 --> 975641
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.83
External database links:  
ASAP:
 ABE-0003134
CGSC:
 31748
ECHOBASE:
 EB2084
ECOLIHUB:
 mukF
OU-MICROARRAY:
 b0922
STRING:
 511145.b0922
COLOMBOS: mukF


Product      
Name: chromosome partitioning protein MukF
Synonym(s): Ca2+-binding protein involved in chromosome partitioning, KicB, MukF
Sequence: Get amino acid sequence Fasta Format
Cellular location: bacterial nucleoid,cytosol
Molecular weight: 50.579
Isoelectric point: 4.507
Motif(s):
 
Type Positions Sequence Comment
3 -> 117 EFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLDGEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYRLTPLGIGITDYYIRQ
121 -> 281 STLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPLKYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLRELQDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIGYDRHV
208 -> 236 LSETSGTLRELQDTLEAAGDKLQANLLRI UniProt: Leucine-zipper; Sequence Annotation Type: region of interest.
233 -> 233 L UniProt: Abolishes function..
283 -> 440 KFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALRDEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRARHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.1 - DNA related
  5 - cell processes --> 5.1 - cell division
  5 - cell processes --> 5.6 - protection --> 5.6.3 - cell killing
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0009295 - nucleoid
GO:0000796 - condensin complex
molecular_function GO:0005515 - protein binding
GO:0005509 - calcium ion binding
biological_process GO:0051301 - cell division
GO:0006260 - DNA replication
GO:0007049 - cell cycle
GO:0007059 - chromosome segregation
GO:0030261 - chromosome condensation
Note(s): Note(s): ...[more].
Reference(s): [1] Svanberg Frisinger F., et al., 2021
External database links:  
ALPHAFOLD:
 P60293
DIP:
 DIP-39981N
ECOCYC:
 EG12165-MONOMER
ECOLIWIKI:
 b0922
INTERPRO:
 IPR036141
INTERPRO:
 IPR033440
INTERPRO:
 IPR036388
INTERPRO:
 IPR036390
INTERPRO:
 IPR038198
INTERPRO:
 IPR005582
INTERPRO:
 IPR033441
INTERPRO:
 IPR033439
MINT:
 P60293
PDB:
 3RPU
PDB:
 1T98
PDB:
 3EUH
PFAM:
 PF17193
PFAM:
 PF17192
PFAM:
 PF03882
PRIDE:
 P60293
PRODB:
 PRO_000023311
REFSEQ:
 NP_415442
SMR:
 P60293
UNIPROT:
 P60293


Operon      
Name: cmoM-mukFEB         
Operon arrangement:
Transcription unit        Promoter
smtA-mukFEB


Transcriptional Regulation      
Display Regulation             
Activated by: H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1194 975868 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1195 979917 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1196 980088 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1197 980108 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Svanberg Frisinger F., Jana B., Donadio S., Guardabassi L., 2021, In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in Escherichia coli., Antibiotics (Basel) 10(6)

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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