RegulonDB RegulonDB 11.1: Gene Form
   

ampG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ampG cyoA yajG Cra Fur ArcA FNR CRP PdhR CRP CusR HprR ampGp4 ampGp4 ampGp6 ampGp6 ampGp5 ampGp5 cyoAp cyoAp

Gene      
Name: ampG    Texpresso search in the literature
Synonym(s): ECK0427, EG12183, b0433
Genome position(nucleotides): 452070 <-- 453545
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.66
External database links:  
ASAP:
 ABE-0001501
CGSC:
 31027
ECHOBASE:
 EB2100
ECOLIHUB:
 ampG
MIM:
 612539
MIM:
 614482
OU-MICROARRAY:
 b0433
STRING:
 511145.b0433
COLOMBOS: ampG


Product      
Name: muropeptide:H+ symporter
Synonym(s): AmpG
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 53.245
Isoelectric point: 8.434
Motif(s):
 
Type Positions Sequence Comment
12 -> 32 PRSAILLILGFASGLPLALTS UniProt: Helical.
17 -> 366 LLILGFASGLPLALTSGTLQAWMTVENIDLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLEPGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSGGLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDFFGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGALYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGTSAFVALLMTLCNKSFSATQFALLSALSAVGRV
48 -> 61 TIGFFSLVGQAYVF UniProt: Helical.
82 -> 105 GWLLATQILLLVAIAAMGFLEPGT UniProt: Helical.
107 -> 124 LRWMAALAVVIAFCSASQ UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.34 - murein turnover, recycling
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
  6 - cell structure --> 6.2 - murein
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015293 - symporter activity
GO:0015295 - solute:proton symporter activity
GO:0022857 - transmembrane transporter activity
biological_process GO:0000270 - peptidoglycan metabolic process
GO:0015835 - peptidoglycan transport
GO:0055085 - transmembrane transport
GO:0071555 - cell wall organization
GO:1902600 - proton transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Perley-Robertson GE., et al., 2016
External database links:  
ALPHAFOLD:
 P0AE16
ECOCYC:
 AMPG-MONOMER
ECOLIWIKI:
 b0433
INTERPRO:
 IPR011701
INTERPRO:
 IPR020846
INTERPRO:
 IPR004752
INTERPRO:
 IPR036259
MODBASE:
 P0AE16
PANTHER:
 PTHR12778
PFAM:
 PF07690
PRIDE:
 P0AE16
PRODB:
 PRO_000022098
PROSITE:
 PS50850
REFSEQ:
 NP_414967
UNIPROT:
 P0AE16


Operon      
Name: yajG-ampG         
Operon arrangement:
Transcription unit        Promoter
yajG
yajG-ampG
ampG


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ampGp5 453627 reverse nd [COMP-AINF] [2]
  promoter ampGp6 453684 reverse nd [COMP-AINF] [2]
  promoter ampGp4 453686 reverse nd [COMP-AINF] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Perley-Robertson GE., Yadav AK., Winogrodzki JL., Stubbs KA., Mark BL., Vocadlo DJ., 2016, A Fluorescent Transport Assay Enables Studying AmpG Permeases Involved in Peptidoglycan Recycling and Antibiotic Resistance., ACS Chem Biol 11(9):2626-35

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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