RegulonDB RegulonDB 11.1: Gene Form
   

glnK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdlB amtB glnK mdlA NtrC TSS_609 TSS_609 glnKp glnKp

Gene      
Name: glnK    Texpresso search in the literature
Synonym(s): ECK0444, EG12191, b0450, ybaI
Genome position(nucleotides): 472598 --> 472936
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.74
External database links:  
ASAP:
 ABE-0001562
ECHOBASE:
 EB2108
ECOLIHUB:
 glnK
OU-MICROARRAY:
 b0450
STRING:
 511145.b0450
COLOMBOS: glnK


Shine dalgarno      
Sequence: aattctgaccGGAGGGgatctATG


Product      
Name: nitrogen regulatory protein PII-2
Synonym(s): GlnK, PII-2, YbaI, nitrogen regulatory protein GlnK
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,inner membrane
Molecular weight: 12.259
Isoelectric point: 5.919
Motif(s):
 
Type Positions Sequence Comment
1 -> 107 MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAELYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRIRTGEA
38 -> 39 RQ UniProt: ADP.
47 -> 47 R R → W: defective in activating NRI-P dephosphoylation in vitro
51 -> 51 Y UniProt: Fully deuridylylated regardless of the N-status of the cell. Still responds to ammonium shock by becoming rapidly sequestered to the membrane. Sequestration rate is significantly more rapid..
87 -> 90 GDGK UniProt: ADP.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.3 - nitrogen metabolism
  3 - regulation --> 3.1 - type of regulation --> 3.1.4 - unknown
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
molecular_function GO:0005515 - protein binding
GO:0030234 - enzyme regulator activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0042802 - identical protein binding
biological_process GO:0050790 - regulation of catalytic activity
GO:0006808 - regulation of nitrogen utilization
GO:0045848 - positive regulation of nitrogen utilization
Evidence: [EXP] Inferred from experiment
Reference(s): [1] Atkinson MR., et al., 2002
[2] Blauwkamp TA., et al., 2002
[3] Radchenko MV., et al., 2013
[4] Radchenko MV., et al., 2014
[5] van Heeswijk WC., et al., 1996
[6] van Heeswijk WC., et al., 1995
External database links:  
ALPHAFOLD:
 P0AC55
DIP:
 DIP-35006N
ECOCYC:
 PROTEIN-PII2
ECOLIWIKI:
 b0450
INTERPRO:
 IPR015867
INTERPRO:
 IPR017918
INTERPRO:
 IPR011322
INTERPRO:
 IPR002187
INTERPRO:
 IPR002332
MODBASE:
 P0AC55
PANTHER:
 PTHR30115
PDB:
 2NUU
PDB:
 2NS1
PDB:
 1GNK
PDB:
 2GNK
PFAM:
 PF00543
PRIDE:
 P0AC55
PRINTS:
 PR00340
PRODB:
 PRO_000022788
PROSITE:
 PS00496
PROSITE:
 PS51343
PROSITE:
 PS00638
REFSEQ:
 NP_414984
SMART:
 SM00938
SMR:
 P0AC55
UNIPROT:
 P0AC55


Operon      
Name: glnK-amtB         
Operon arrangement:
Transcription unit        Promoter
glnK-amtB


Transcriptional Regulation      
Display Regulation             
Activated by: Fur, FNR, GadX, NtrC
Repressed by: FNR, Fur


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_609 472557 forward nd [RS-EPT-CBR] [7]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Atkinson MR., Blauwkamp TA., Ninfa AJ., 2002, Context-dependent functions of the PII and GlnK signal transduction proteins in Escherichia coli., J Bacteriol 184(19):5364-75

 [2] Blauwkamp TA., Ninfa AJ., 2002, Physiological role of the GlnK signal transduction protein of Escherichia coli: survival of nitrogen starvation., Mol Microbiol 46(1):203-14

 [3] Radchenko MV., Thornton J., Merrick M., 2013, P(II) signal transduction proteins are ATPases whose activity is regulated by 2-oxoglutarate., Proc Natl Acad Sci U S A 110(32):12948-53

 [4] Radchenko MV., Thornton J., Merrick M., 2014, Association and dissociation of the GlnK-AmtB complex in response to cellular nitrogen status can occur in the absence of GlnK post-translational modification., Front Microbiol 5:731

 [5] van Heeswijk WC., Hoving S., Molenaar D., Stegeman B., Kahn D., Westerhoff HV., 1996, An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli., Mol Microbiol 21(1):133-46

 [6] van Heeswijk WC., Stegeman B., Hoving S., Molenaar D., Kahn D., Westerhoff HV., 1995, An additional PII in Escherichia coli: a new regulatory protein in the glutamine synthetase cascade., FEMS Microbiol Lett 132(1-2):153-7

 [7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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