RegulonDB

Gene
Name:	iscX
Synonym(s):	ECK2521, EG12311, b2524, yfhJ
Genome position(nucleotides):	2656536 <-- 2656736 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.25
External database links:

ASAP:	ABE-0008309
ECHOBASE:	EB2217
ECOLIHUB:	iscX
OU-MICROARRAY:	b2524
STRING:	511145.b2524
COLOMBOS:	iscX

Product

Name:

accessory iron-sulfur cluster assembly protein IscX

Synonym(s):

IscX, YfhJ, regulator of iron-sulfur cluster assembly

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

7.732

Isoelectric point:

3.719

Motif(s):

Type	Positions	Sequence
	43 -> 51	DDDPQASNE
	58 -> 66	LLVWLDEAE
	55 -> 55	E
	3 -> 66	LKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDDDPQASNEKILEAILLVWLDEAE

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0004857 - enzyme inhibitor activity GO:0005506 - iron ion binding GO:0008198 - ferrous iron binding
	biological_process	GO:0016226 - iron-sulfur cluster assembly GO:0043086 - negative regulation of catalytic activity

Note(s):

Note(s): ...[more].

Evidence:

[IDA] Inferred from direct assay

Reference(s):

[1] Kim JH., et al., 2014
[2] Nakamura M., et al., 1999
[3] Shi R., et al., 2010
[4] Tanaka N., et al., 2016

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PANTHER:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

hscBA-fdx-iscX

Operon arrangement:

Transcription unit	Promoter
hscBA-fdx-iscX	hscBp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2823	2656567	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2824	2656727	reverse	nd	[RS-EPT-CBR]	[5]
promoter	iscXp3	2656917	reverse	nd	[ICWHO]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Kim JH., Bothe JR., Frederick RO., Holder JC., Markley JL., 2014, Role of IscX in iron-sulfur cluster biogenesis in Escherichia coli., J Am Chem Soc 136(22):7933-42
[2] Nakamura M., Saeki K., Takahashi Y., 1999, Hyperproduction of recombinant ferredoxins in escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA-hscB-hs cA-fdx-ORF3 gene cluster., J Biochem (Tokyo) 126(1):10-8
[3] Shi R., Proteau A., Villarroya M., Moukadiri I., Zhang L., Trempe JF., Matte A., Armengod ME., Cygler M., 2010, Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by IscS protein-protein interactions., PLoS Biol 8(4):e1000354
[4] Tanaka N., Kanazawa M., Tonosaki K., Yokoyama N., Kuzuyama T., Takahashi Y., 2016, Novel features of the ISC machinery revealed by characterization of Escherichia coli mutants that survive without iron-sulfur clusters., Mol Microbiol 99(5):835-48
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78