RegulonDB RegulonDB 11.1: Gene Form
   

clcA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

clcA yadW yadX erpA IscR PdhR RyhB TSS_300 TSS_300 erpAp erpAp TSS_298 TSS_298 TSS_297 TSS_297 TSS_296 TSS_296 clcAp10 clcAp10

Gene      
Name: clcA    Texpresso search in the literature
Synonym(s): ECK0154, EG12331, b0155, eriC, yadQ
Genome position(nucleotides): 175107 --> 176528
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.87
External database links:  
ASAP:
 ABE-0000533
ECHOBASE:
 EB2235
ECOLIHUB:
 clcA
MIM:
 308990
MIM:
 300554
MIM:
 300009
MIM:
 310468
OU-MICROARRAY:
 b0155
STRING:
 511145.b0155
COLOMBOS: clcA


Product      
Name: chloride:H+ antiporter ClcA
Synonym(s): ClC-ec1, ClcA, EriC, YadQ
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 50.349
Isoelectric point: 9.794
Motif(s):
 
Type Positions Sequence Comment
32 -> 32 P UniProt: In Ref. 1..
33 -> 69 LAILFMAAVVGTLVGLAAVAFDKGVAWLQNQRMGALV UniProt: Helical.
77 -> 100 LLLTVAFLCSAVLAMFGYFLVRKY UniProt: Helical.
91 -> 436 MFGYFLVRKYAPEAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGRMVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAVFIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGMQDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLVFIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIAGMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLA
106 -> 110 GSGIP UniProt: Selectivity filter part_1; Sequence Annotation Type: short sequence motif.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.1 - Channel-type Transporters --> 4.1.A - alpha-type channels
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0062158 - chloride:proton antiporter activity
GO:0015297 - antiporter activity
GO:0015108 - chloride transmembrane transporter activity
GO:0005247 - voltage-gated chloride channel activity
GO:0015299 - solute:proton antiporter activity
GO:0042802 - identical protein binding
biological_process GO:0006811 - ion transport
GO:0006821 - chloride transport
GO:0055085 - transmembrane transport
GO:1902600 - proton transmembrane transport
GO:1902476 - chloride transmembrane transport
GO:1990451 - cellular stress response to acidic pH
External database links:  
ALPHAFOLD:
 P37019
DIP:
 DIP-9523N
ECOCYC:
 YADQ-MONOMER
ECOLIWIKI:
 b0155
INTERPRO:
 IPR023861
INTERPRO:
 IPR014743
INTERPRO:
 IPR001807
MODBASE:
 P37019
PDB:
 2FEE
PDB:
 2H2P
PDB:
 2H2S
PDB:
 2HLF
PDB:
 2HT2
PDB:
 2HT3
PDB:
 2HT4
PDB:
 2HTK
PDB:
 2HTL
PDB:
 2R9H
PDB:
 3DET
PDB:
 3EJY
PDB:
 3EJZ
PDB:
 3NMO
PDB:
 4ENE
PDB:
 4FTP
PDB:
 4KJP
PDB:
 4KJQ
PDB:
 4KJW
PDB:
 4KK5
PDB:
 4KK6
PDB:
 4KK8
PDB:
 4KK9
PDB:
 4KKA
PDB:
 4KKB
PDB:
 4KKC
PDB:
 4KKL
PDB:
 4LOU
PDB:
 4MQX
PDB:
 5HD8
PDB:
 6AD7
PDB:
 6AD8
PDB:
 6ADA
PDB:
 6ADB
PDB:
 6ADC
PDB:
 6K5D
PDB:
 6K5F
PDB:
 6LSC
PDB:
 6V2J
PDB:
 2FED
PDB:
 2FEC
PDB:
 2EZ0
PDB:
 2EXY
PDB:
 2EXW
PDB:
 1OTU
PDB:
 1OTT
PDB:
 1OTS
PDB:
 1KPK
PFAM:
 PF00654
PRIDE:
 P37019
PRINTS:
 PR00762
PRODB:
 PRO_000022294
REFSEQ:
 NP_414697
SMR:
 P37019
UNIPROT:
 P37019


Operon      
Name: clcA         
Operon arrangement:
Transcription unit        Promoter
clcA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter clcAp10 175052 forward nd [COMP-AINF] [1]
  promoter TSS_296 175074 forward nd [RS-EPT-CBR] [2]
  promoter TSS_297 176147 forward nd [RS-EPT-CBR] [2]
  promoter TSS_298 176558 forward nd [RS-EPT-CBR] [2]
  promoter TSS_300 176583 forward nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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