RegulonDB RegulonDB 10.10: Gene Form
   

menF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

menD menF elaB menH terminator TSS_2522 TSS_2522 TSS_2521 TSS_2521 TSS_2520 TSS_2520

Gene      
Name: menF    Texpresso search in the literature
Synonym(s): ECK2259, EG12362, b2265, yfbA
Genome position(nucleotides): 2379348 <-- 2380643 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.4
External database links:  
ASAP:
 ABE-0007485
CGSC:
 35955
ECHOBASE:
 EB2265
ECOLIHUB:
 menF
OU-MICROARRAY:
 b2265
STRING:
 511145.b2265
COLOMBOS: menF


Product      
Name: isochorismate synthase MenF
Synonym(s): MenF, YfbA, isochorismate synthase 2
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 48.765
Isoelectric point: 7.102
Motif(s):
 
Type Positions Sequence
255 -> 255 L
310 -> 312 VLR
344 -> 344 A
190 -> 190 K
240 -> 240 E

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.11 - menaquinone, ubiquinone
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016853 - isomerase activity
GO:0046872 - metal ion binding
GO:0008909 - isochorismate synthase activity
GO:0000287 - magnesium ion binding
biological_process GO:0009058 - biosynthetic process
GO:0009234 - menaquinone biosynthetic process
External database links:  
DIP:
 DIP-10187N
ECOCYC:
 MENF-MONOMER
ECOLIWIKI:
 b2265
INTERPRO:
 IPR004561
INTERPRO:
 IPR005801
INTERPRO:
 IPR015890
INTERPRO:
 IPR034681
MODBASE:
 P38051
PDB:
 3BZN
PDB:
 3BZM
PDB:
 2EUA
PFAM:
 PF00425
PRIDE:
 P38051
PRODB:
 PRO_000023205
REFSEQ:
 NP_416768
SMR:
 P38051
UNIPROT:
 P38051


Operon      
Name: menFDHBCE         
Operon arrangement:
Transcription unit        Promoter
menD-yfbB-menBCE
menBCE
menFD-yfbB-menBCE


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2520 2379245 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2521 2379281 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2522 2380846 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB