RegulonDB RegulonDB 11.1: Gene Form
   

mdtK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdtK ydhQ ribC terminator anti-terminator anti-anti-terminator TSS_1954 TSS_1954 ribCp5 ribCp5 mdtKp9 mdtKp9 mdtKp9 mdtKp9 TSS_1953 TSS_1953 mdtKp10 mdtKp10

Gene      
Name: mdtK    Texpresso search in the literature
Synonym(s): ECK1659, EG12400, b1663, norE, norM, ydhE
Genome position(nucleotides): 1743457 --> 1744830
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.98
External database links:  
ASAP:
 ABE-0005561
ECHOBASE:
 EB2300
ECOLIHUB:
 mdtK
OU-MICROARRAY:
 b1663
STRING:
 511145.b1663
COLOMBOS: mdtK


Product      
Name: multidrug efflux pump MdtK
Synonym(s): MdtK, NorE, NorM, YdhE
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 49.447
Isoelectric point: 9.897
Motif(s):
 
Type Positions Sequence Comment
11 -> 31 LLALAIPVILAQIAQTAMGFV UniProt: Helical.
17 -> 177 PVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYI
32 -> 32 D UniProt: In Ref. 1; AAB47941..
53 -> 73 IWLPAILFGHGLLLALTPVIA UniProt: Helical.
93 -> 113 WLAGFVSVLIMLVLWNAGYII UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015297 - antiporter activity
GO:0015298 - solute:cation antiporter activity
GO:0022857 - transmembrane transporter activity
GO:0042910 - xenobiotic transmembrane transporter activity
GO:0071916 - dipeptide transmembrane transporter activity
biological_process GO:1990961 - xenobiotic detoxification by transmembrane export across the plasma membrane
GO:0006811 - ion transport
GO:0046677 - response to antibiotic
GO:0006814 - sodium ion transport
GO:0015031 - protein transport
GO:0006855 - xenobiotic transmembrane transport
GO:0015833 - peptide transport
GO:0055085 - transmembrane transport
GO:0034614 - cellular response to reactive oxygen species
GO:0042908 - xenobiotic transport
GO:0035442 - dipeptide transmembrane transport
GO:0098655 - cation transmembrane transport
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P37340
ECOCYC:
 YDHE-MONOMER
ECOLIWIKI:
 b1663
INTERPRO:
 IPR022913
INTERPRO:
 IPR002528
PFAM:
 PF01554
PRIDE:
 P37340
PRODB:
 PRO_000023191
REFSEQ:
 YP_025307
UNIPROT:
 P37340


Operon      
Name: mdtK         
Operon arrangement:
Transcription unit        Promoter
mdtK


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter mdtKp10 1743269 forward nd [COMP-AINF] [1]
  promoter TSS_1953 1743275 reverse nd [RS-EPT-CBR] [2]
  promoter mdtKp9 1743375 forward nd [COMP-AINF] [1]
  promoter mdtKp9 1743375 forward nd [COMP-AINF] [1]
  promoter ribCp5 1743458 reverse nd [COMP-AINF] [1]
  promoter TSS_1954 1744638 forward nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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