RegulonDB RegulonDB 10.9: Gene Form
   

mlaE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mlaF mlaE mlaD TSS_3504 TSS_3504 TSS_3503 TSS_3503 TSS_3502 TSS_3502

Gene      
Name: mlaE    Texpresso search in the literature
Synonym(s): ECK3183, EG12800, b3194, yrbE
Genome position(nucleotides): 3338466 <-- 3339248 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.17
External database links:  
ASAP:
ABE-0010495
ECHOBASE:
EB2652
ECOLIHUB:
mlaE
OU-MICROARRAY:
b3194
STRING:
511145.b3194
COLOMBOS: mlaE


Product      
Name: intermembrane phospholipid transport system, integral membrane subunit MlaE
Synonym(s): MlaE, YrbE
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 27.863
Isoelectric point: 10.511
Motif(s):
 
Type Positions Sequence
148 -> 168 FWAGVISLPLLTVIFVAVGIW
89 -> 109 MLVALSLLRELGPVVAALLFA
199 -> 219 LVNCLIKSVVFAITVTWISLF
239 -> 259 VVHSSLAVLGLDFVLTALMFG
45 -> 256 VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTVIFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISLFNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTAL

 

Classification:
Multifun Terms (GenProtEC)  
  6 - cell structure --> 6.1 - membrane
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.m - ABC superfamily, membrane component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0032991 - protein-containing complex
GO:0016021 - integral component of membrane
GO:0043190 - ATP-binding cassette (ABC) transporter complex
molecular_function GO:0120014 - phospholipid transfer activity
GO:0005548 - phospholipid transporter activity
biological_process GO:0120010 - intermembrane phospholipid transfer
GO:0015914 - phospholipid transport
Note(s): Note(s): ...[more].
Reference(s): [1] Ekiert DC., et al., 2017
[2] Hughes GW., et al., 2019
External database links:  
ECOCYC:
YRBE-MONOMER
ECOLIWIKI:
b3194
INTERPRO:
IPR030802
INTERPRO:
IPR003453
PANTHER:
PTHR30188
PFAM:
PF02405
PRIDE:
P64606
REFSEQ:
NP_417661
UNIPROT:
P64606


Operon      
Name: mlaFEDCB         
Operon arrangement:
Transcription unit        Promoter
mlaFEDCB
mlaFEDCB
mlaFEDCB


Transcriptional Regulation      
Display Regulation             
Activated by: MarA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3502 3337936 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3503 3338185 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3504 3338787 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Ekiert DC., Bhabha G., Isom GL., Greenan G., Ovchinnikov S., Henderson IR., Cox JS., Vale RD., 2017, Architectures of Lipid Transport Systems for the Bacterial Outer Membrane., Cell 169(2):273-285.e17

 [2] Hughes GW., Hall SCL., Laxton CS., Sridhar P., Mahadi AH., Hatton C., Piggot TJ., Wotherspoon PJ., Leney AC., Ward DG., Jamshad M., Spana V., Cadby IT., Harding C., Isom GL., Bryant JA., Parr RJ., Yakub Y., Jeeves M., Huber D., Henderson IR., Clifton LA., Lovering AL., Knowles TJ., 2019, Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system., Nat Microbiol 4(10):1692-1705

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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