RegulonDB RegulonDB 11.1: Gene Form
   

pta gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pta yfcC ackA SdhX terminator anti-terminator anti-anti-terminator terminator TSS_2612 TSS_2612 TSS_2611 TSS_2611 TSS_2610 TSS_2610 TSS_2609 TSS_2609 TSS_2608 TSS_2608 TSS_2607 TSS_2607 TSS_2606 (cluster) TSS_2606 (cluster) TSS_2605 TSS_2605 TSS_2604 TSS_2604 ptap ptap TSS_2602 TSS_2602 TSS_2601 TSS_2601 TSS_2600 TSS_2600 TSS_2599 TSS_2599 TSS_2598 TSS_2598 TSS_2597 TSS_2597 TSS_2596 TSS_2596 TSS_2595 TSS_2595 TSS_2594 TSS_2594 TSS_2593 TSS_2593

Gene      
Name: pta    Texpresso search in the literature
Synonym(s): ECK2291, EG20173, b2297
Genome position(nucleotides): 2414747 --> 2416891
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.52
External database links:  
ASAP:
 ABE-0007582
CGSC:
 353
ECHOBASE:
 EB4147
ECOLIHUB:
 pta
OU-MICROARRAY:
 b2297
STRING:
 511145.b2297
COLOMBOS: pta


Product      
Name: phosphate acetyltransferase
Synonym(s): Pta
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 77.172
Isoelectric point: 5.054
Motif(s):
 
Type Positions Sequence Comment
2 -> 714 SRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPRTGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDAEVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFGGAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVLGAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSLLVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNTNTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAFRYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGVELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDGLVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQLAEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGPLQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQGMRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ UniProt: Phosphate acetyltransferase.
4 -> 230 IIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPRTGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDAEVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFGGAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVLGAVPWSFDLIAT
20 -> 50 SLGVIRAMERKGVRLSVFKPIAQPRTGGDAP UniProt: In Ref. 2; BAA04663..
208 -> 209 KL UniProt: In Ref. 1; BAA04502 and 2; BAA04663..
234 -> 345 DMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSLLVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNTNTWQTSLSLQS

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.28 - pyruvate oxidation
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.29 - acetate catabolism
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.30 - threonine catabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0016407 - acetyltransferase activity
GO:0016746 - acyltransferase activity
GO:0008270 - zinc ion binding
GO:0008959 - phosphate acetyltransferase activity
biological_process GO:0006083 - acetate metabolic process
GO:0006085 - acetyl-CoA biosynthetic process
GO:0045733 - acetate catabolic process
GO:0019413 - acetate biosynthetic process
GO:0019427 - acetyl-CoA biosynthetic process from acetate
GO:0070689 - L-threonine catabolic process to propionate
Note(s): Note(s): ...[more].
Reference(s): [1] Gupta S., et al., 1989
[2] Pascal MC., et al., 1981
[3] Yang YT., et al., 1999
External database links:  
ALPHAFOLD:
 P0A9M8
DIP:
 DIP-35815N
ECOCYC:
 PHOSACETYLTRANS-MONOMER
ECOLIWIKI:
 b2297
INTERPRO:
 IPR010766
INTERPRO:
 IPR027417
INTERPRO:
 IPR016475
INTERPRO:
 IPR042112
INTERPRO:
 IPR042113
INTERPRO:
 IPR002505
INTERPRO:
 IPR004614
INTERPRO:
 IPR028979
MODBASE:
 P0A9M8
PFAM:
 PF13500
PFAM:
 PF01515
PFAM:
 PF07085
PRIDE:
 P0A9M8
PRODB:
 PRO_000023627
REFSEQ:
 NP_416800
SMR:
 P0A9M8
UNIPROT:
 P0A9M8


Operon      
Name: ackA-pta         
Operon arrangement:
Transcription unit        Promoter
ackA
pta
ackA-pta


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2593 2413470 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2594 2413474 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2595 2413477 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2596 2413482 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2597 2413792 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2598 2413797 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2599 2413805 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2600 2413957 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2601 2414269 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2602 2414360 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2604 2415098 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2605 2415351 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2606 (cluster) 2415354 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2607 2415750 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2608 2415781 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2609 2415814 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2610 2415818 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2611 2416038 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2612 2416149 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Gupta S., Clark DP., 1989, Escherichia coli derivatives lacking both alcohol dehydrogenase and phosphotransacetylase grow anaerobically by lactate fermentation., J Bacteriol 171(7):3650-5

 [2] Pascal MC., Chippaux M., Abou-Jaoude A., Blaschkowski HP., Knappe J., 1981, Mutants of Escherichia coli K12 with defects in anaerobic pyruvate metabolism., J Gen Microbiol 124(1):35-42

 [3] Yang YT., Bennett GN., San KY., 1999, Effect of inactivation of nuo and ackA-pta on redistribution of metabolic fluxes in Escherichia coli., Biotechnol Bioeng 65(3):291-7

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB