RegulonDB RegulonDB 11.1: Gene Form
   

metZ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mltA metZ metW ppGpp anti-anti-terminator anti-terminator terminator TSS_3124 TSS_3124 TSS_3123 TSS_3123 metZp metZp mltAp6 mltAp6 TSS_3122 TSS_3122

Gene      
Name: metZ    Texpresso search in the literature
Synonym(s): ECK2810, EG30062, b2814, metZalpha
Genome position(nucleotides): 2947387 --> 2947463
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 66.23
External database links:  
ASAP:
 ABE-0009232
CGSC:
 503
ECHOBASE:
 EB4225
ECOLIHUB:
 metZ
OU-MICROARRAY:
 b2814
COLOMBOS: metZ


Product      
Name: tRNA-initiator Met(CAU)
Synonym(s): tRNAfMet1, tRNAmetZ
Type: tRNAs
Cellular location: cytosol
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.5 - tRNA
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0030533 - triplet codon-amino acid adaptor activity
Note(s): Note(s): ...[more].
Evidence: [AS-TAS] Traceable author statement
Reference(s): [1] Shetty S., et al., 2014
[2] Shetty S., et al., 2016
[3] Siekevitz P., et al., 1981
External database links:  
ECOCYC:
 metZ-tRNA
ECOLIWIKI:
 b2814


Operon      
Name: metZWV         
Operon arrangement:
Transcription unit        Promoter
metZWV


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3122 2946548 reverse nd [RS-EPT-CBR] [4]
  promoter mltAp6 2947273 reverse nd [COMP-AINF] [5]
  promoter TSS_3123 2947497 forward nd [RS-EPT-CBR] [4]
  promoter TSS_3124 2947518 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Shetty S., Nadimpalli H., Shah RA., Arora S., Das G., Varshney U., 2014, An extended Shine-Dalgarno sequence in mRNA functionally bypasses a vital defect in initiator tRNA., Proc Natl Acad Sci U S A 111(40):E4224-33

 [2] Shetty S., Varshney U., 2016, An evolutionarily conserved element in initiator tRNAs prompts ultimate steps in ribosome maturation., Proc Natl Acad Sci U S A 113(41):E6126-E6134

 [3] Siekevitz P., Zamecnik PC., 1981, Ribosomes and protein synthesis., J Cell Biol 91(3 Pt 2):53s-65s

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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