RegulonDB RegulonDB 10.9: Gene Form
   

rpmA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yhbE rplU rpmA terminator TSS_3494 TSS_3494 TSS_3493 TSS_3493 rpmAp4 rpmAp4 TSS_3492 TSS_3492

Gene      
Name: rpmA    Texpresso search in the literature
Synonym(s): ECK3174, EG50002, b3185, rpz
Genome position(nucleotides): 3332862 <-- 3333119 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.0
External database links:  
ASAP:
 ABE-0010468
CGSC:
 241
ECHOBASE:
 EB4296
ECOLIHUB:
 rpmA
OU-MICROARRAY:
 b3185
STRING:
 511145.b3185
COLOMBOS: rpmA


Product      
Name: 50S ribosomal subunit protein L27
Synonym(s): RpmA, Rpz
Sequence: Get amino acid sequence Fasta Format
Cellular location: ribosome,cytosol
Molecular weight: 9.124
Isoelectric point: 11.33
Motif(s):
 
Type Positions Sequence
2 -> 82 AHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISI

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
  2 - information transfer --> 2.3 - protein related --> 2.3.8 - ribosomal proteins
  6 - cell structure --> 6.6 - ribosomes
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0005840 - ribosome
GO:0022625 - cytosolic large ribosomal subunit
molecular_function GO:0003735 - structural constituent of ribosome
GO:0005515 - protein binding
GO:0043022 - ribosome binding
GO:0003723 - RNA binding
GO:0000049 - tRNA binding
GO:0019843 - rRNA binding
biological_process GO:1902626 - assembly of large subunit precursor of preribosome
GO:0090070 - positive regulation of ribosome biogenesis
GO:0006412 - translation
GO:0000027 - ribosomal large subunit assembly
GO:0042256 - mature ribosome assembly
GO:0001558 - regulation of cell growth
Note(s): Note(s): ...[more].
Reference(s): [1] Amarantos I., et al., 2001
[2] Kirillov SV., et al., 2002
[3] Maguire BA., et al., 2001
[4] Wittmann-Liebold B., et al., 1995
[5] Wower J., et al., 1995
[6] Xiao M., et al., 2012
External database links:  
DIP:
 DIP-47933N
DISPROT:
 DP00140
ECOCYC:
 EG50002-MONOMER
ECOLIWIKI:
 b3185
INTERPRO:
 IPR001684
INTERPRO:
 IPR018261
PANTHER:
 PTHR15893
PDB:
 2J28
PDB:
 2RDO
PDB:
 3BBX
PDB:
 3J5L
PDB:
 3J7Z
PDB:
 3J8G
PDB:
 3J9Y
PDB:
 3J9Z
PDB:
 3JA1
PDB:
 3JBU
PDB:
 3JBV
PDB:
 3JCD
PDB:
 3JCE
PDB:
 3JCJ
PDB:
 3JCN
PDB:
 4CSU
PDB:
 4U1U
PDB:
 4U1V
PDB:
 4U20
PDB:
 4U24
PDB:
 4U25
PDB:
 4U26
PDB:
 4U27
PDB:
 4UY8
PDB:
 4V47
PDB:
 4V48
PDB:
 4V4H
PDB:
 4V4Q
PDB:
 4V4V
PDB:
 4V4W
PDB:
 4V50
PDB:
 4V52
PDB:
 4V53
PDB:
 4V54
PDB:
 4V55
PDB:
 4V56
PDB:
 4V57
PDB:
 4V5B
PDB:
 4V5H
PDB:
 4V5Y
PDB:
 4V64
PDB:
 4V65
PDB:
 4V66
PDB:
 4V69
PDB:
 4V6C
PDB:
 4V6D
PDB:
 4V6E
PDB:
 4V6K
PDB:
 4V6L
PDB:
 4V6M
PDB:
 4V6N
PDB:
 4V6O
PDB:
 4V6P
PDB:
 4V6Q
PDB:
 4V6R
PDB:
 4V6S
PDB:
 4V6T
PDB:
 4V6V
PDB:
 4V6Y
PDB:
 4V6Z
PDB:
 4V70
PDB:
 4V71
PDB:
 4V72
PDB:
 4V73
PDB:
 4V74
PDB:
 4V75
PDB:
 4V76
PDB:
 4V77
PDB:
 4V78
PDB:
 4V79
PDB:
 4V7A
PDB:
 4V7B
PDB:
 4V7C
PDB:
 4V7D
PDB:
 4V7I
PDB:
 4V7S
PDB:
 4V7T
PDB:
 4V7U
PDB:
 4V7V
PDB:
 4V85
PDB:
 4V89
PDB:
 4V9C
PDB:
 4V9D
PDB:
 4V9O
PDB:
 4V9P
PDB:
 4WF1
PDB:
 4WOI
PDB:
 4WWW
PDB:
 4YBB
PDB:
 5ADY
PDB:
 5AFI
PDB:
 5AKA
PDB:
 5GAD
PDB:
 5GAE
PDB:
 5GAF
PDB:
 5GAG
PDB:
 5GAH
PDB:
 5H5U
PDB:
 5IQR
PDB:
 5IT8
PDB:
 5J5B
PDB:
 5J7L
PDB:
 5J88
PDB:
 5J8A
PDB:
 5J91
PDB:
 5JC9
PDB:
 5JTE
PDB:
 5JU8
PDB:
 5KCR
PDB:
 5KCS
PDB:
 5KPS
PDB:
 5KPV
PDB:
 5KPW
PDB:
 5KPX
PDB:
 5L3P
PDB:
 5LZA
PDB:
 5LZB
PDB:
 5LZC
PDB:
 5LZD
PDB:
 5LZE
PDB:
 5LZF
PDB:
 5MDV
PDB:
 5MDW
PDB:
 5MDY
PDB:
 5MDZ
PDB:
 5MGP
PDB:
 5NCO
PDB:
 5NP6
PDB:
 5NWY
PDB:
 5O2R
PDB:
 5U4I
PDB:
 5U9F
PDB:
 5U9G
PDB:
 5UYK
PDB:
 5UYL
PDB:
 5UYM
PDB:
 5UYN
PDB:
 5UYP
PDB:
 5UYQ
PDB:
 5WDT
PDB:
 5WE4
PDB:
 5WE6
PDB:
 5WFK
PDB:
 6BU8
PDB:
 6BY1
PDB:
 6C4H
PDB:
 6C4I
PDB:
 6ENF
PDB:
 6ENJ
PDB:
 6ENU
PDB:
 6GBZ
PDB:
 6GC0
PDB:
 6GC4
PDB:
 6GC8
PDB:
 6GWT
PDB:
 6GXM
PDB:
 6GXN
PDB:
 6GXO
PDB:
 6GXP
PDB:
 6H4N
PDB:
 6H58
PDB:
 6HRM
PDB:
 6I0Y
PDB:
 6I7V
PDB:
 6O9J
PDB:
 6O9K
PDB:
 6OFX
PDB:
 6OG7
PDB:
 6OGF
PDB:
 6OGG
PDB:
 6OGI
PDB:
 6ORE
PDB:
 6ORL
PDB:
 6OST
PDB:
 6OT3
PDB:
 6OUO
PDB:
 6Q97
PDB:
 6Q98
PDB:
 6Q9A
PDB:
 6QUL
PDB:
 6S0K
PDB:
 6SZS
PDB:
 6TBV
PDB:
 6TC3
PFAM:
 PF01016
PRIDE:
 P0A7L8
PRINTS:
 PR00063
PRODB:
 PRO_000023832
PROSITE:
 PS00831
REFSEQ:
 NP_417652
SMR:
 P0A7L8
UNIPROT:
 P0A7L8


Operon      
Name: rplU-rpmA-yhbE-obgE         
Operon arrangement:
Transcription unit        Promoter
rplU-rpmA
rplU-rpmA-yhbE-obgE
yhbE-obgE


Transcriptional Regulation      
Display Regulation             
Activated by: MlrA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3492 3333224 reverse nd [RS-EPT-CBR] [7]
  promoter rpmAp4 3333225 reverse Similarity to the consensus
Read more > 		[ICWHO] [8]
  promoter TSS_3493 3333320 reverse nd [RS-EPT-CBR] [7]
  promoter TSS_3494 3333375 reverse nd [RS-EPT-CBR] [7]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Amarantos I., Xaplanteri MA., Choli-Papadopoulou T., Kalpaxis DL., 2001, Effects of two photoreactive spermine analogues on peptide bond formation and their application for labeling proteins in Escherichia coli functional ribosomal complexes., Biochemistry 40(25):7641-50

 [2] Kirillov SV., Wower J., Hixson SS., Zimmermann RA., 2002, Transit of tRNA through the Escherichia coli ribosome: cross-linking of the 3' end of tRNA to ribosomal proteins at the P and E sites., FEBS Lett 514(1):60-6

 [3] Maguire BA., Manuilov AV., Zimmermann RA., 2001, Differential effects of replacing Escherichia coli ribosomal protein L27 with its homologue from Aquifex aeolicus., J Bacteriol 183(22):6565-72

 [4] Wittmann-Liebold B., Uhlein M., Urlaub H., Muller EC., Otto A., Bischof O., 1995, Structural and functional implications in the eubacterial ribosome as revealed by protein-rRNA and antibiotic contact sites., Biochem Cell Biol 73(11-12):1187-97

 [5] Wower J., Wower IK., Kirillov SV., Rosen KV., Hixson SS., Zimmermann RA., 1995, Peptidyl transferase and beyond., Biochem Cell Biol 73(11-12):1041-7

 [6] Xiao M., Wang Y., 2012, L27-tRNA interaction revealed by mutagenesis and pH titration., Biophys Chem 167:8-15

 [7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [8] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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