RegulonDB RegulonDB 10.10: Gene Form
   

cybC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

Gene      
Name: cybC    Texpresso search in the literature
Synonym(s): ECK4231, EG50008, b4236, cytochrome b562
Type: Pseudo Gene
Genome position(nucleotides): 4459488 --> 4459855 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.46
Note(s): The protein product of cybC was not identified in the periplasmic proteome of E. coli K-12 MG1655 Han MJ, Kim JY, Kim JA,2014. The nucleotide sequence of cybC from the K-12 strain NM522 contains a 26 bp deletion when compared to the sequence of cybC from E. coli B which encodes soluble cytochrome b562. The deletion occurs at the 5' end of the gene and extends into the putative signal sequence Trower MK,1993. Cytochrome b-562 could not be detected in cells carrying the K strain gene expressed from a plasmid Trower MK,1993.
Comment from ASAP:
The coordinates of cybC have been changed from those reported in the GenBank entry (GBAN:U00096.2) to correspond to the region of similarity to Escherichia coli Sakai (ASAP:ACA-0135330), adding 65 nucleotides to the N-terminus.
Reference(s): [1] Itagaki E., et al., 1968
[2] Mathews FS., et al., 1979
[3] Nikkila H., et al., 1991
External database links:  
ASAP:
 ABE-0013858
CGSC:
 34583
ECHOBASE:
 EB4301
ECOLIHUB:
 cybC
ECOLIWIKI:
 b4236
OU-MICROARRAY:
 b4236
UNIPROT:
 P0ABE7
COLOMBOS: cybC


Operon      
Name: cybC         
Operon arrangement:
Transcription unit        Promoter
cybC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yjgAp8 4458015 reverse nd [ICWHO] [4]
  promoter yjgAp11 4458046 reverse nd [ICWHO] [4]
  promoter yjgAp9 4458065 reverse nd [ICWHO] [4]
  promoter yjgAp5 4458081 reverse nd [ICWHO] [4]


Evidence    

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Itagaki E., Hager LP., 1968, The amino acid sequence of cytochrome b562 of Escherichia coli., Biochem Biophys Res Commun 32(6):1013-9

 [2] Mathews FS., Bethge PH., Czerwinski EW., 1979, The structure of cytochrome b562 from Escherichia coli at 2.5 A resolution., J Biol Chem 254(5):1699-706

 [3] Nikkila H., Gennis RB., Sligar SG., 1991, Cloning and expression of the gene encoding the soluble cytochrome b562 of Escherichia coli., Eur J Biochem 202(2):309-13

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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