RegulonDB RegulonDB 10.10: Gene Form
   

ecnA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

efp ecnB ecnA OmpR anti-anti-terminator anti-terminator terminator terminator ecnBp ecnBp TSS_4965 (cluster) TSS_4965 (cluster) epmBp3 epmBp3 TSS_4964 TSS_4964

Gene      
Name: ecnA    Texpresso search in the literature
Synonym(s): ECK4142, G0-41, b4410
Genome position(nucleotides): 4376317 --> 4376442 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.97
Reference(s): [1] Bishop RE., et al., 1998
External database links:  
ASAP:
 ABE-0047210
ECHOBASE:
 EB4115
ECOLIHUB:
 ecnA
STRING:
 511145.b4410
COLOMBOS: ecnA


Product      
Name: entericidin A lipoprotein, antidote to entericidin B
Synonym(s): EcnA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,outer membrane
Molecular weight: 4.359
Isoelectric point: 10.76
Motif(s):
 
Type Positions Sequence
1 -> 18 MMKRLIVLVLLASTLLTG
21 -> 40 TARGFGEDIKHLGNSISRAA

 
Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0036405 - anchored component of cell outer membrane
biological_process GO:0009636 - response to toxic substance
Note(s): Note(s): ...[more].
Evidence: [AUPEINH] Assay of unpurified protein expressed in its native host
Reference(s): [2] Alix E., et al., 2009
[1] Bishop RE., et al., 1998
[3] Gonnet P., et al., 2004
[4] Rao S., et al., 2020
External database links:  
ECOCYC:
 MONOMER0-1562
ECOLIWIKI:
 b4410
INTERPRO:
 IPR012556
PFAM:
 PF08085
PRIDE:
 P0ADB4
PROSITE:
 PS51257
REFSEQ:
 YP_026283
UNIPROT:
 P0ADB4


Operon      
Name: ecnAB         
Operon arrangement:
Transcription unit        Promoter
ecnB
ecnAB


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4964 4375700 reverse nd [RS-EPT-CBR] [5]
  promoter epmBp3 4375703 reverse nd [ICWHO] [6]
  promoter TSS_4965 (cluster) 4375964 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Bishop RE., Leskiw BK., Hodges RS., Kay CM., Weiner JH., 1998, The entericidin locus of Escherichia coli and its implications for programmed bacterial cell death., J Mol Biol 280(4):583-96

 [2] Alix E., Blanc-Potard AB., 2009, Hydrophobic peptides: novel regulators within bacterial membrane., Mol Microbiol 72(1):5-11

 [3] Gonnet P., Rudd KE., Lisacek F., 2004, Fine-tuning the prediction of sequences cleaved by signal peptidase II: a curated set of proven and predicted lipoproteins of Escherichia coli K-12., Proteomics 4(6):1597-613

 [4] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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