RegulonDB

Gene
Name:	micA
Synonym(s):	ECK2682, G0-8866, b4442, psrA10, sraD
Genome position(nucleotides):	2814802 --> 2814874
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	42.47
Reference(s):	[1] Argaman L., et al., 2001
External database links:

ASAP:	ABE-0047262
ECHOBASE:	EB4528
ECOLIHUB:	micA
COLOMBOS:	micA

Product

Name:

small regulatory RNA MicA

Synonym(s):

MicA, PsrA10, SraD

Type:

small RNA

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.7 - antisense RNA
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.6 - antisense RNA
Gene Ontology Terms (GO)
	molecular_function	GO:0005515 - protein binding GO:0003729 - mRNA binding
	biological_process	GO:0006950 - response to stress GO:0040033 - RNA-mediated gene silencing by inhibition of translation GO:1902021 - regulation of bacterial-type flagellum-dependent cell motility

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IDA] Inferred from direct assay
[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[2] Guo MS., et al., 2014
[3] Hammann P., et al., 2014
[4] Mihailovic MK., et al., 2018
[5] Moores A., et al., 2014
[6] Nagamitsu H., et al., 2013
[7] Rasmussen AA., et al., 2005
[8] Udekwu KI. 2010
[9] Udekwu KI., et al., 2005
[10] Vincent HA., et al., 2013
[11] Zhang S., et al., 2018

External database links:

ECOCYC:

SRAD-RNA

ECOLIWIKI:

b4442

Operon

Name:

micA

Operon arrangement:

Transcription unit	Promoter
micA	micAp

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2998	2815236	reverse	nd	[RS-EPT-CBR]	[12]
promoter	TSS_2999	2815242	reverse	nd	[RS-EPT-CBR]	[12]
promoter	TSS_3000	2816260	reverse	nd	[RS-EPT-CBR]	[12]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Argaman L., Hershberg R., Vogel J., Bejerano G., Wagner EG., Margalit H., Altuvia S., 2001, Novel small RNA-encoding genes in the intergenic regions of Escherichia coli., Curr Biol 11(12):941-50
[2] Guo MS., Updegrove TB., Gogol EB., Shabalina SA., Gross CA., Storz G., 2014, MicL, a new σE-dependent sRNA, combats envelope stress by repressing synthesis of Lpp, the major outer membrane lipoprotein., Genes Dev 28(14):1620-34
[3] Hammann P., Parmentier D., Cerciat M., Reimegard J., Helfer AC., Boisset S., Guillier M., Vandenesch F., Wagner EG., Romby P., Fechter P., 2014, A method to map changes in bacterial surface composition induced by regulatory RNAs in Escherichia coli and Staphylococcus aureus., Biochimie 106:175-9
[4] Mihailovic MK., Vazquez-Anderson J., Li Y., Fry V., Vimalathas P., Herrera D., Lease RA., Powell WB., Contreras LM., 2018, High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites., Nat Commun 9(1):4084
[5] Moores A., Chipper-Keating S., Sun L., McVicker G., Wales L., Gashi K., Blomfield IC., 2014, RfaH Suppresses Small RNA MicA Inhibition of fimB Expression in Escherichia coli K-12., J Bacteriol 196(1):148-56
[6] Nagamitsu H., Murata M., Kosaka T., Kawaguchi J., Mori H., Yamada M., 2013, Crucial roles of MicA and RybB as vital factors for σ-dependent cell lysis in Escherichia coli long-term stationary phase., J Mol Microbiol Biotechnol 23(3):227-32
[7] Rasmussen AA., Eriksen M., Gilany K., Udesen C., Franch T., Petersen C., Valentin-Hansen P., 2005, Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control., Mol Microbiol 58(5):1421-9
[8] Udekwu KI., 2010, Transcriptional and post-transcriptional regulation of the Escherichia coli luxS mRNA; involvement of the sRNA MicA., PLoS One 5(10):e13449
[9] Udekwu KI., Darfeuille F., Vogel J., Reimegard J., Holmqvist E., Wagner EG., 2005, Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA., Genes Dev 19(19):2355-66
[10] Vincent HA., Phillips JO., Henderson CA., Roberts AJ., Stone CM., Mardle CE., Butt LE., Gowers DM., Pickford AR., Callaghan AJ., 2013, An improved method for surface immobilisation of RNA: application to small non-coding RNA-mRNA pairing., PLoS One 8(11):e79142
[11] Zhang S., Liu S., Wu N., Yuan Y., Zhang W., Zhang Y., 2018, Small Non-coding RNA RyhB Mediates Persistence to Multiple Antibiotics and Stresses in Uropathogenic Escherichia coli by Reducing Cellular Metabolism., Front Microbiol 9:136
[12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.