RegulonDB

Gene
Name:	mgtS
Synonym(s):	ECK4409, G0-8892, b4599, yneM
Genome position(nucleotides):	1622646 --> 1622741
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	38.54
External database links:

ECOLIHUB:	yneM
STRING:	511145.b4599
COLOMBOS:	mgtS

Product

Name:

small protein MgtS

Synonym(s):

MgtS, YneM

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

3.509

Isoelectric point:

5.41

Motif(s):

Type	Positions	Sequence	Comment
	4 -> 26	NMNVFMAVLGIILFSGFLAAYFS
	30 -> 30	D	D → A: loss-of-function mutation, expressed at wild-type levels (see also \|CITS: [30276893]\|)
	31 -> 31	D	D → A: loss-of-function mutation

Classification:

Multifun Terms (GenProtEC)
		5 - cell processes --> 5.5 - adaptations --> 5.5.3 - starvation
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0005515 - protein binding
	biological_process	GO:0010960 - magnesium ion homeostasis GO:0010350 - cellular response to magnesium starvation

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IGI] Inferred from genetic interaction
[EXP-IMP] Inferred from mutant phenotype
[EXP-IPI] Inferred from physical interaction

Reference(s):

[1] Duval M., et al., 2017
[2] Wang H., et al., 2017

External database links:

ALPHAFOLD:

ECOCYC:

ECOLIWIKI:

PDB:

PDB:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

mgtS

Operon arrangement:

Transcription unit	Promoter
mgtS	mgtSp

Transcriptional Regulation
	Display Regulation
Activated by:	PhoP

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_1843	1622587		forward	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Duval M., Cossart P., 2017, Small bacterial and phagic proteins: an updated view on a rapidly moving field., Curr Opin Microbiol 39:81-88
[2] Wang H., Yin X., Wu Orr M., Dambach M., Curtis R., Storz G., 2017, Increasing intracellular magnesium levels with the 31-amino acid MgtS protein., Proc Natl Acad Sci U S A 114(22):5689-5694
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.