RegulonDB RegulonDB 10.9: Gene Form
   

tisB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

istR tisA tisB istR-1 ysdE LexA  IstR-2  IstR-1  IstR-2  IstR-1 terminator anti-anti-terminator anti-terminator anti-anti-terminator anti-terminator terminator TSS_4252 TSS_4252 tisBp tisBp istR-2p istR-2p istR-1p istR-1p

Gene      
Name: tisB    Texpresso search in the literature
Synonym(s): ECK4427, G0-9962, b4618, ysdB
Genome position(nucleotides): 3853553 --> 3853642 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
42.22
External database links:  
ECOLIHUB:
tisB
STRING:
511145.b4618
COLOMBOS: tisB


Product      
Name: membrane-depolarizing toxin TisB
Synonym(s): TisB, YsdB, toxic peptide TisB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 3.222
Isoelectric point: 9.224
Motif(s):
 
Type Positions Sequence
1 -> 27 MNLVDIAILILKLIVAALQLLDAVLKY
3 -> 25 LVDIAILILKLIVAALQLLDAVL

 

Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.8 - SOS response
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005253 - anion channel activity
biological_process GO:0006974 - cellular response to DNA damage stimulus
GO:0009432 - SOS response
GO:0015698 - inorganic anion transport
GO:0022611 - dormancy process
Note(s): Note(s): ...[more].
Evidence: [IMP] Inferred from mutant phenotype
Reference(s): [1] Berghoff BA., et al., 2017
[2] Dorr T., et al., 2010
[3] Vogel J., et al., 2004
External database links:  
ECOCYC:
MONOMER0-1922
ECOLIWIKI:
b4618
INTERPRO:
IPR025211
PFAM:
PF13939
PRIDE:
A5A627
REFSEQ:
YP_001165331
UNIPROT:
A5A627


Operon      
Name: tisB         
Operon arrangement:
Transcription unit        Promoter
tisB


Transcriptional Regulation      
Display Regulation             
Repressed by: LexA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4252 3853442 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Berghoff BA., Hoekzema M., Aulbach L., Wagner EG., 2017, Two regulatory RNA elements affect TisB-dependent depolarization and persister formation., Mol Microbiol 103(6):1020-1033

 [2] Dorr T., Vulic M., Lewis K., 2010, Ciprofloxacin causes persister formation by inducing the TisB toxin in Escherichia coli., PLoS Biol 8(2):e1000317

 [3] Vogel J., Argaman L., Wagner EG., Altuvia S., 2004, The small RNA IstR inhibits synthesis of an SOS-induced toxic peptide., Curr Biol 14(24):2271-6

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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