RegulonDB RegulonDB 10.9: Gene Form
   

fadH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ygjK fadH higA higB ArcA FadR CRP HipB fadHp fadHp

Gene      
Name: fadH    Texpresso search in the literature
Synonym(s): ECK3071, G36, b3081, ygjL
Genome position(nucleotides): 3231665 --> 3233683 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 57.21
Reference(s): [1] He XY., et al., 1997
External database links:  
ASAP:
 ABE-0010124
ECHOBASE:
 EB2582
ECOLIHUB:
 fadH
OU-MICROARRAY:
 b3081
STRING:
 511145.b3081
COLOMBOS: fadH


Shine dalgarno      
Sequence: ttttacatacTGGACAattttATG


Product      
Name: 2,4-dienoyl-CoA reductase
Synonym(s): FadH, YgjL
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 72.678
Isoelectric point: 6.547
Motif(s):
 
Type Positions Sequence
2 -> 672 SYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFYAERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQILHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVEVMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSMLDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKLKGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINTCIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAINAAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVTADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGFDTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKASKPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVICAGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI
656 -> 658 LDA
563 -> 564 RK
165 -> 165 E
5 -> 331 SLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFYAERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQILHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVEVMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSMLDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKLKGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADE

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.2 - fatty acids
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.4 - fatty acids and phosphatidic acid
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0008670 - 2,4-dienoyl-CoA reductase (NADPH) activity
GO:0010181 - FMN binding
GO:0071949 - FAD binding
biological_process GO:0006635 - fatty acid beta-oxidation
GO:0055114 - oxidation-reduction process
GO:0033543 - fatty acid beta-oxidation, unsaturated, even number, reductase/isomerase pathway
Note(s): Note(s): ...[more].
Reference(s): [2] Kimura C., et al., 2004
[3] Mizugaki M., et al., 1983
[4] Mizugaki M., et al., 1982
[5] Mizugaki M., et al., 1982
External database links:  
DIP:
 DIP-9562N
ECOCYC:
 DIENOYLCOAREDUCT-MONOMER
ECOLIWIKI:
 b3081
INTERPRO:
 IPR001155
INTERPRO:
 IPR036188
INTERPRO:
 IPR023753
INTERPRO:
 IPR013785
MODBASE:
 P42593
PDB:
 1PS9
PFAM:
 PF07992
PFAM:
 PF00724
PRIDE:
 P42593
PRODB:
 PRO_000022570
REFSEQ:
 NP_417552
SMR:
 P42593
UNIPROT:
 P42593


Operon      
Name: fadH         
Operon arrangement:
Transcription unit        Promoter
fadH


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: FadR, HipB, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] He XY., Yang SY., Schulz H., 1997, Cloning and expression of the fadH gene and characterization of the gene product 2,4-dienoyl coenzyme A reductase from Escherichia coli., Eur J Biochem 248(2):516-20

 [2] Kimura C., Mizugaki M., Yamanaka H., Fujino M., Morishima T., 2004, [2,4-Dienoyl-CoA reductases: from discovery toward pathophysiological significance]., Nihon Rinsho 62(8):1577-83

 [3] Mizugaki M., Kimura C., Nishimaki T., Kawaguchi A., Okuda S., Yamanaka H., 1983, Studies on the metabolism of unsaturated fatty acids. XII. Reaction catalyzed by 2,4-dienoyl-CoA reductase of Escherichia coli., J Biochem 94(2):409-13

 [4] Mizugaki M., Kimura C., Nishimaki T., Yamamoto H., Sagi M., Nishimura S., Yamanaka H., 1982, Studies on the metabolism of unsaturated fatty acids. X. Purification and some properties of 2,4-dienoyl-CoA reductase from Escherichia coli., J Biochem 92(5):1671-4

 [5] Mizugaki M., Nishimaki T., Yamamoto H., Nishimura S., Sagi M., Yamanaka H., 1982, Studies on the metabolism of unsaturated fatty acids. VIII. Induction of 2,4-dienoyl-CoA reductase in Escherichia coli on the addition of unsaturated fatty acids., J Biochem 91(4):1453-6

RegulonDB