RegulonDB RegulonDB 11.1: Gene Form
   

insE2 gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

insEF2 insF2 iprA insE2 yaiVp4 yaiVp4 TSS_488 TSS_488 insFp4 insFp4 insFp5 insFp5

Gene      
Name: insE2    Texpresso search in the literature
Synonym(s): ECK0298, G6224, b0373
Genome position(nucleotides): 392602 <-- 392901
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.0
External database links:  
ASAP:
 ABE-0001279
ECOLIHUB:
 insE-2
OU-MICROARRAY:
 b0373
STRING:
 511145.b2088
COLOMBOS: insE2


Product      
Name: IS3 element protein InsE
Synonym(s): InsE2
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 11.543
Isoelectric point: 10.57
Motif(s):
 
Type Positions Sequence Comment
1 -> 21 MTKTVSTSKKPRKQHSPEFRS UniProt: Disordered; Sequence Annotation Type: region of interest.
12 -> 78 RKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSSERELEMSTEIARLKRQLA

 
Classification:
Multifun Terms (GenProtEC)  
  8 - extrachromosomal --> 8.3 - transposon related
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0004803 - transposase activity
biological_process GO:0006310 - DNA recombination
GO:0006313 - transposition, DNA-mediated
GO:0032196 - transposition
External database links:  
ALPHAFOLD:
 P0CF67
ECOCYC:
 MONOMER0-4242
ECOLIWIKI:
 b0373
INTERPRO:
 IPR002514
INTERPRO:
 IPR009057
PFAM:
 PF01527
PRIDE:
 P0CF67
REFSEQ:
 NP_414907
UNIPROT:
 P0CF67


Operon      
Name: insEF2E2F2         
Operon arrangement:
Transcription unit        Promoter
insE2F2EF2


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter insFp5 392619 reverse nd [COMP-AINF] [1]
  promoter insFp4 392660 reverse nd [COMP-AINF] [1]
  promoter TSS_488 392979 reverse nd [RS-EPT-CBR] [2]
  promoter yaiVp4 394461 forward nd [COMP-AINF] [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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