RegulonDB

Gene
Name:	yajO
Synonym(s):	ECK0413, G6236, b0419
Genome position(nucleotides):	437161 <-- 438135
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.03
External database links:

ASAP:	ABE-0001457
ECHOBASE:	EB3377
ECOLIHUB:	yajO
OU-MICROARRAY:	b0419
STRING:	511145.b0419
COLOMBOS:	yajO

Product

Name:

1-deoxyxylulose-5-phosphate synthase YajO

Synonym(s):

YajO

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

36.42

Isoelectric point:

4.965

Motif(s):

Type	Positions	Sequence	Comment
	15 -> 312	RLCLGCMTFGEPDRGNHAWTLPEESSRPIIKRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSRAQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMHASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGRLTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSKPGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELE

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0016491 - oxidoreductase activity
	biological_process	GO:0006772 - thiamine metabolic process GO:1902767 - isoprenoid biosynthetic process via mevalonate

Note(s):

Note(s): ...[more].

Evidence:

[EXP] Inferred from experiment
[EXP-IGI-FUNC-COMPLEMENTATION] Inferred by functional complementation

Reference(s):

[1] Kirby J., et al., 2015
[2] Lawhorn BG., et al., 2004
[3] Spindler EC., et al., 2013

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PRIDE:

PRINTS:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

xseB-ispA-dxs-yajO

Operon arrangement:

Transcription unit	Promoter
xseB-ispA-dxs-yajO	xseBp
yajO	yajOp

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_537	438284		reverse	nd	[RS-EPT-CBR]	[4]
	promoter	TSS_538 (cluster)	439919		reverse	nd	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Kirby J., Nishimoto M., Chow RW., Baidoo EE., Wang G., Martin J., Schackwitz W., Chan R., Fortman JL., Keasling JD., 2015, Enhancing Terpene yield from sugars via novel routes to 1-deoxy-d-xylulose 5-phosphate., Appl Environ Microbiol 81(1):130-8
[2] Lawhorn BG., Gerdes SY., Begley TP., 2004, A genetic screen for the identification of thiamin metabolic genes., J Biol Chem 279(42):43555-9
[3] Spindler EC., Boyle NR., Hancock RE., Gill RT., 2013, Genome-wide identification of genes conferring energy related resistance to a synthetic antimicrobial peptide (Bac8c)., PLoS One 8(1):e55052
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.