RegulonDB RegulonDB 11.1: Gene Form
   

mscK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mscK acrR ybaM SoxS MarA anti-terminator terminator TSS_631 TSS_631 TSS_630 TSS_630 TSS_629 TSS_629 mscKp mscKp TSS_628 TSS_628 TSS_627 TSS_627 TSS_626 (cluster) TSS_626 (cluster)

Gene      
Name: mscK    Texpresso search in the literature
Synonym(s): ECK0459, G6255, aefA, b0465, kefA
Genome position(nucleotides): 486536 --> 489898
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.89
External database links:  
ASAP:
 ABE-0001613
ECHOBASE:
 EB3991
ECOLIHUB:
 kefA
OU-MICROARRAY:
 b0465
STRING:
 511145.b0465
COLOMBOS: mscK


Product      
Name: potassium dependent, small conductance mechanosensitive channel
Synonym(s): AefA, KefA, MscK
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 127.215
Isoelectric point: 8.2
Motif(s):
 
Type Positions Sequence Comment
1 -> 33 MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFA
34 -> 1120 RASSNGDLPTKADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKMRQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVSLQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQRKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPDEAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKEQIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAFVNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSVSKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWQKAWPAVFIAFLAGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLIILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTSHWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRESWRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQTVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLMFALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVAWALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQWLAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTITDFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHPRVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAFNQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG UniProt: Mechanosensitive channel MscK.
50 -> 289 QLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKMRQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVSLQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQRKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPDEAARIQANPLVKQ
500 -> 520 WPAVFIAFLAGLPLLLIAGLI UniProt: Helical.
509 -> 826 AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLIILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTSHWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRESWRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQTVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLMFALFGVMFWAIWSDLITVFSYLDSITLW

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.1 - Channel-type Transporters --> 4.1.A - alpha-type channels
  5 - cell processes --> 5.5 - adaptations --> 5.5.6 - other (mechanical, nutritional, oxidative stress)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0008381 - mechanosensitive ion channel activity
biological_process GO:0006811 - ion transport
GO:0006813 - potassium ion transport
GO:0009992 - cellular water homeostasis
GO:0034220 - ion transmembrane transport
GO:0055085 - transmembrane transport
GO:0035864 - response to potassium ion
Note(s): Note(s): ...[more].
Reference(s): [1] Akitake B., et al., 2005
[2] Berrier C., et al., 1989
[3] Cox CD., et al., 2013
[4] Hurst AC., et al., 2009
[5] Miller S., et al., 2003
[6] Petrov E., et al., null
External database links:  
ALPHAFOLD:
 P77338
DIP:
 DIP-10070N
ECOCYC:
 G6255-MONOMER
ECOLIWIKI:
 b0465
INTERPRO:
 IPR023408
INTERPRO:
 IPR010920
INTERPRO:
 IPR006685
INTERPRO:
 IPR024393
INTERPRO:
 IPR025692
INTERPRO:
 IPR023298
INTERPRO:
 IPR011066
INTERPRO:
 IPR011014
INTERPRO:
 IPR006686
MODBASE:
 P77338
PFAM:
 PF12795
PFAM:
 PF00924
PFAM:
 PF12794
PRIDE:
 P77338
PRODB:
 PRO_000023060
PROSITE:
 PS01246
REFSEQ:
 NP_414998
SMR:
 P77338
UNIPROT:
 P77338


Operon      
Name: mscK         
Operon arrangement:
Transcription unit        Promoter
mscK


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_626 (cluster) 485980 reverse nd [RS-EPT-CBR] [7]
  promoter TSS_627 486041 reverse nd [RS-EPT-CBR] [7]
  promoter TSS_628 486086 reverse nd [RS-EPT-CBR] [7]
  promoter TSS_629 487911 forward nd [RS-EPT-CBR] [7]
  promoter TSS_630 488317 forward nd [RS-EPT-CBR] [7]
  promoter TSS_631 489636 forward nd [RS-EPT-CBR] [7]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Akitake B., Anishkin A., Sukharev S., 2005, The "dashpot" mechanism of stretch-dependent gating in MscS., J Gen Physiol 125(2):143-54

 [2] Berrier C., Coulombe A., Houssin C., Ghazi A., 1989, A patch-clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes. Pressure-activated channels are localized in the inner membrane., FEBS Lett 259(1):27-32

 [3] Cox CD., Nomura T., Ziegler CS., Campbell AK., Wann KT., Martinac B., 2013, Selectivity mechanism of the mechanosensitive channel MscS revealed by probing channel subconducting states., Nat Commun 4:2137

 [4] Hurst AC., Gottlieb PA., Martinac B., 2009, Concentration dependent effect of GsMTx4 on mechanosensitive channels of small conductance in E. coli spheroplasts., Eur Biophys J 38(4):415-25

 [5] Miller S., Bartlett W., Chandrasekaran S., Simpson S., Edwards M., Booth IR., 2003, Domain organization of the MscS mechanosensitive channel of Escherichia coli., EMBO J 22(1):36-46

 [6] Petrov E., Rohde PR., Cornell B., Martinac B., null, The protective effect of osmoprotectant TMAO on bacterial mechanosensitive channels of small conductance MscS/MscK under high hydrostatic pressure., Channels (Austin) 6(4):262-71

 [7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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