RegulonDB RegulonDB 11.1: Gene Form
   

cusC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cusC cusR cusF cusS CusR HprR PhoB terminator anti-terminator anti-anti-terminator TSS_714 TSS_714 TSS_713 TSS_713 TSS_712 (cluster) TSS_712 (cluster) TSS_711 TSS_711 TSS_710 (cluster) TSS_710 (cluster) TSS_709 TSS_709 TSS_708 TSS_708 TSS_707 TSS_707 TSS_706 TSS_706 TSS_705 TSS_705 TSS_704 TSS_704 TSS_703 TSS_703 TSS_702 TSS_702 TSS_701 (cluster) TSS_701 (cluster) TSS_700 TSS_700 TSS_699 (cluster) TSS_699 (cluster) TSS_698 TSS_698 cusCp cusCp cusRp cusRp TSS_697 TSS_697

Gene      
Name: cusC    Texpresso search in the literature
Synonym(s): ECK0564, G6320, b0572, ibeB, ylcB
Genome position(nucleotides): 595600 --> 596973
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.24
External database links:  
ASAP:
 ABE-0001958
ECHOBASE:
 EB3984
ECOLIHUB:
 cusC
OU-MICROARRAY:
 b0572
STRING:
 511145.b0572
COLOMBOS: cusC


Product      
Name: copper/silver export system outer membrane channel
Synonym(s): CusC, IbeB, YlcB
Sequence: Get amino acid sequence Fasta Format
Cellular location: outer membrane
Molecular weight: 50.27
Isoelectric point: 6.313
Motif(s):
 
Type Positions Sequence Comment
1 -> 17 MSPCKLLPFCVALALTG
6 -> 6 L UniProt: In strain: K1 / RS218..
18 -> 457 CSLAPDYQRPAMPVPQQFSLSQNGLVNAADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQLNGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRAVHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQARGVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQILLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNFIPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQISAQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISLYTALGGG UniProt: Cation efflux system protein CusC.
20 -> 20 L UniProt: In strain: K1 / RS218..
27 -> 27 P UniProt: In strain: K1 / RS218..

 
Classification:
Multifun Terms (GenProtEC)  
  6 - cell structure --> 6.1 - membrane
  8 - extrachromosomal --> 8.3 - transposon related
Gene Ontology Terms (GO)  
cellular_component GO:0009279 - cell outer membrane
GO:0016020 - membrane
GO:0016021 - integral component of membrane
GO:0046930 - pore complex
molecular_function GO:0005507 - copper ion binding
GO:0005515 - protein binding
GO:0015288 - porin activity
GO:0005375 - copper ion transmembrane transporter activity
GO:0015562 - efflux transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
GO:0042802 - identical protein binding
GO:0019992 - diacylglycerol binding
biological_process GO:0006811 - ion transport
GO:0046688 - response to copper ion
GO:0009636 - response to toxic substance
GO:0006878 - cellular copper ion homeostasis
GO:0055085 - transmembrane transport
GO:0010272 - response to silver ion
GO:0010273 - detoxification of copper ion
GO:0060003 - copper ion export
GO:0035434 - copper ion transmembrane transport
GO:0018345 - protein palmitoylation
GO:0070207 - protein homotrimerization
GO:1902601 - silver ion transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Rao S., et al., 2020
[2] Xu Z., et al., 2019
External database links:  
ALPHAFOLD:
 P77211
DIP:
 DIP-9347N
ECOCYC:
 G6320-MONOMER
ECOLIWIKI:
 b0572
INTERPRO:
 IPR010131
INTERPRO:
 IPR003423
MODBASE:
 P77211
PDB:
 4K7R
PDB:
 3PIK
PDB:
 4K7K
PDB:
 4K34
PFAM:
 PF02321
PRIDE:
 P77211
PRODB:
 PRO_000022351
PROSITE:
 PS51257
REFSEQ:
 NP_415104
SMR:
 P77211
SWISSMODEL:
 P77211
UNIPROT:
 P77211


Operon      
Name: cusCFBA         
Operon arrangement:
Transcription unit        Promoter
cusCFBA


Transcriptional Regulation      
Display Regulation             
Activated by: HprR, PhoB, CusR


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_697 595461 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_698 595574 forward nd [RS-EPT-CBR] [3]
  promoter TSS_699 (cluster) 595588 forward nd [RS-EPT-CBR] [3]
  promoter TSS_700 595625 forward nd [RS-EPT-CBR] [3]
  promoter TSS_701 (cluster) 595826 forward nd [RS-EPT-CBR] [3]
  promoter TSS_702 596004 forward nd [RS-EPT-CBR] [3]
  promoter TSS_703 596425 forward nd [RS-EPT-CBR] [3]
  promoter TSS_704 596427 forward nd [RS-EPT-CBR] [3]
  promoter TSS_705 596430 forward nd [RS-EPT-CBR] [3]
  promoter TSS_706 596539 forward nd [RS-EPT-CBR] [3]
  promoter TSS_707 596568 forward nd [RS-EPT-CBR] [3]
  promoter TSS_708 596571 forward nd [RS-EPT-CBR] [3]
  promoter TSS_709 596810 forward nd [RS-EPT-CBR] [3]
  promoter TSS_710 (cluster) 596903 forward nd [RS-EPT-CBR] [3]
  promoter TSS_711 596918 forward nd [RS-EPT-CBR] [3]
  promoter TSS_712 (cluster) 596941 forward nd [RS-EPT-CBR] [3]
  promoter TSS_713 596946 forward nd [RS-EPT-CBR] [3]
  promoter TSS_714 597240 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3

 [2] Xu Z., Wang P., Wang H., Yu ZH., Au-Yeung HY., Hirayama T., Sun H., Yan A., 2019, Zinc excess increases cellular demand for iron and decreases tolerance to copper in Escherichia coli., J Biol Chem 294(45):16978-16991

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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