RegulonDB RegulonDB 11.1: Gene Form
   

ybiW gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ybiW ybiY ybiV ybiWp4 ybiWp4 ybiVp5 ybiVp5

Gene      
Name: ybiW    Texpresso search in the literature
Synonym(s): ECK0813, G6426, b0823, pflF
Genome position(nucleotides): 860174 <-- 862606
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.99
External database links:  
ASAP:
 ABE-0002810
ECHOBASE:
 EB3112
ECOLIHUB:
 ybiW
OU-MICROARRAY:
 b0823
STRING:
 511145.b0823
COLOMBOS: ybiW


Product      
Name: putative pyruvate formate lyase
Synonym(s): PflF, YbiW
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 90.125
Isoelectric point: 5.817
Motif(s):
 
Type Positions Sequence Comment
10 -> 671 SDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDGLREEVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGHDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFG
11 -> 682 DRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDGLREEVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGHDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK UniProt: PFL.
677 -> 699 TPDGRKAHTPLAEGASPASGTDH UniProt: Disordered; Sequence Annotation Type: region of interest.
688 -> 791 AEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFF
689 -> 810 EGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTEHML UniProt: Glycine radical.

 
Classification:
Multifun Terms (GenProtEC)  
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
Note(s): Note(s): ...[more].
Reference(s): [1] Paradis-Bleau C., et al., 2014
External database links:  
ALPHAFOLD:
 P75793
ECOCYC:
 G6426-MONOMER
ECOLIWIKI:
 b0823
INTERPRO:
 IPR010098
INTERPRO:
 IPR019777
INTERPRO:
 IPR004184
INTERPRO:
 IPR001150
MODBASE:
 P75793
PFAM:
 PF02901
PFAM:
 PF01228
PRIDE:
 P75793
PROSITE:
 PS00850
PROSITE:
 PS51554
PROSITE:
 PS51149
REFSEQ:
 NP_415344
SMR:
 P75793
UNIPROT:
 P75793


Operon      
Name: ybiYW         
Operon arrangement:
Transcription unit        Promoter
ybiYW


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybiVp5 860088 reverse nd [COMP-AINF] [2]
  promoter ybiWp4 862783 reverse nd [COMP-AINF] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Paradis-Bleau C., Kritikos G., Orlova K., Typas A., Bernhardt TG., 2014, A genome-wide screen for bacterial envelope biogenesis mutants identifies a novel factor involved in cell wall precursor metabolism., PLoS Genet 10(1):e1004056

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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