RegulonDB RegulonDB 11.1: Gene Form
   

mdtH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdtH grxB rimJ ppGpp terminator yceHp2 yceHp2 yceHp4 yceHp4 rimJp rimJp TSS_1339 TSS_1339 TSS_1338 TSS_1338 grxBp grxBp grxBp2 grxBp2 grxBp4 grxBp4 TSS_1337 (cluster) TSS_1337 (cluster)

Gene      
Name: mdtH    Texpresso search in the literature
Synonym(s): ECK1050, G6559, b1065, yceL
Genome position(nucleotides): 1124118 <-- 1125326
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.33
External database links:  
ASAP:
 ABE-0003618
ECHOBASE:
 EB3637
ECOLIHUB:
 mdtH
OU-MICROARRAY:
 b1065
STRING:
 511145.b1065
COLOMBOS: mdtH


Product      
Name: multidrug efflux pump MdtH
Synonym(s): MdtH, YceL
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 44.363
Isoelectric point: 10.467
Motif(s):
 
Type Positions Sequence Comment
13 -> 33 YFLLIDNMLVVLGFFVVFPLI UniProt: Helical.
20 -> 239 MLVVLGFFVVFPLISIRFVDQMGWAAVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLLWFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQYDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLAGYYMLAVQVMLMLPIMVNDVA
99 -> 116 PWLLWFSCLLSGLGGTLF UniProt: Helical.
139 -> 159 LLMMQDSAGAVIGALLGSWLL UniProt: Helical.
165 -> 185 LVCATGAVLFVLCAAFNAWLL UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0022857 - transmembrane transporter activity
biological_process GO:0046677 - response to antibiotic
GO:0055085 - transmembrane transport
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P69367
ECOCYC:
 B1065-MONOMER
ECOLIWIKI:
 b1065
INTERPRO:
 IPR022855
INTERPRO:
 IPR020846
INTERPRO:
 IPR036259
INTERPRO:
 IPR011701
MODBASE:
 P69367
PFAM:
 PF07690
PRIDE:
 P69367
PRODB:
 PRO_000023188
PROSITE:
 PS50850
REFSEQ:
 NP_415583
UNIPROT:
 P69367


Operon      
Name: mdtH         
Operon arrangement:
Transcription unit        Promoter
mdtH


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1337 (cluster) 1124081 reverse nd [RS-EPT-CBR] [1]
  promoter grxBp4 1124083 reverse nd [COMP-AINF] [2]
  promoter grxBp2 1124102 reverse nd [COMP-AINF] [2]
  promoter TSS_1338 1125354 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1339 1125475 forward nd [RS-EPT-CBR] [1]
  promoter yceHp4 1126077 forward nd [COMP-AINF] [2]
  promoter yceHp2 1126081 forward nd [COMP-AINF] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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