RegulonDB RegulonDB 10.9: Gene Form
   

thiK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nagZ thiK lpoB ycfL TSS_1438 TSS_1438 TSS_1437 TSS_1437 TSS_1436 TSS_1436

Gene      
Name: thiK    Texpresso search in the literature
Synonym(s): ECK1092, G6566, b1106, ycfN
Genome position(nucleotides): 1163260 --> 1164084 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.33
External database links:  
ASAP:
ABE-0003735
ECHOBASE:
EB3206
ECOLIHUB:
thiK
OU-MICROARRAY:
b1106
STRING:
511145.b1106
COLOMBOS: thiK


Product      
Name: thiamine kinase
Synonym(s): ThiK, YcfN
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.397
Isoelectric point: 10.16
Motif(s):
 
Type Positions Sequence
25 -> 222 VTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAK

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.8 - thiamin
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0019165 - thiamine kinase activity
biological_process GO:0006772 - thiamine metabolic process
GO:0016310 - phosphorylation
GO:0009229 - thiamine diphosphate biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ECOCYC:
THIKIN-MONOMER
ECOLIWIKI:
b1106
INTERPRO:
IPR011009
INTERPRO:
IPR014093
INTERPRO:
IPR002575
MODBASE:
P75948
PFAM:
PF01636
PRIDE:
P75948
PRODB:
PRO_000024060
REFSEQ:
NP_415624
UNIPROT:
P75948


Operon      
Name: hinT-ycfL-lpoB-thiK-nagZ-ycfP         
Operon arrangement:
Transcription unit        Promoter
hinT-ycfL-lpoB-thiK-nagZ-ycfP


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1436 1163603 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1437 1164943 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1438 1165016 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


RegulonDB