RegulonDB RegulonDB 11.1: Gene Form
   

ttcA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dbpA intR ttcA TSS_1774 TSS_1774 TSS_1773 (cluster) TSS_1773 (cluster) TSS_1772 TSS_1772 TSS_1771 TSS_1771 ttcAp5 ttcAp5 TSS_1770 TSS_1770

Gene      
Name: ttcA    Texpresso search in the literature
Synonym(s): ECK1341, G6675, b1344, ydaO
Genome position(nucleotides): 1411013 <-- 1411948
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 48.61
External database links:  
ASAP:
 ABE-0004514
ECHOBASE:
 EB3140
ECOLIHUB:
 ttcA
OU-MICROARRAY:
 b1344
STRING:
 511145.b1344
COLOMBOS: ttcA


Product      
Name: tRNA cytosine32 2-sulfurtransferase TtcA
Synonym(s): TtcA, YdaO, tRNA C32 thiolase
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 35.561
Isoelectric point: 6.262
Motif(s):
 
Type Positions Sequence Comment
11 -> 11 E UniProt: In Ref. 3; BAA04675..
42 -> 204 IMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKA
45 -> 45 C UniProt: Able to functionally complement a ttcA deletion mutant as efficiently as wild-type..
47 -> 52 SGGKDS UniProt: PP-loop motif; Sequence Annotation Type: short sequence motif.
122 -> 122 C UniProt: Not able to functionally complement a ttcA deletion mutant..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0003723 - RNA binding
GO:0016783 - sulfurtransferase activity
GO:0000049 - tRNA binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0000287 - magnesium ion binding
GO:0042803 - protein homodimerization activity
biological_process GO:0006400 - tRNA modification
GO:0008033 - tRNA processing
GO:0034227 - tRNA thio-modification
External database links:  
ALPHAFOLD:
 P76055
ECOCYC:
 G6675-MONOMER
ECOLIWIKI:
 b1344
INTERPRO:
 IPR011063
INTERPRO:
 IPR014729
INTERPRO:
 IPR012089
INTERPRO:
 IPR035107
MODBASE:
 P76055
PFAM:
 PF01171
PRIDE:
 P76055
PRODB:
 PRO_000024136
REFSEQ:
 NP_415860
SMR:
 P76055
UNIPROT:
 P76055


Operon      
Name: ttcA         
Operon arrangement:
Transcription unit        Promoter
ttcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1770 1411438 reverse nd [RS-EPT-CBR] [1]
  promoter ttcAp5 1411985 reverse nd [COMP-AINF] [2]
  promoter TSS_1771 1412059 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1772 1412113 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1773 (cluster) 1412117 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1774 1412124 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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