RegulonDB RegulonDB 11.1: Gene Form
   

tcyP gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tcyP ydjO ydjM terminator TSS_2045 (cluster) TSS_2045 (cluster) tcyPp tcyPp

Gene      
Name: tcyP    Texpresso search in the literature
Synonym(s): ECK1727, G6934, b1729, ydjN
Genome position(nucleotides): 1810934 --> 1812325
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.44
External database links:  
ASAP:
 ABE-0005771
ECHOBASE:
 EB3746
ECOLIHUB:
 ydjN
OU-MICROARRAY:
 b1729
STRING:
 511145.b1729
COLOMBOS: tcyP


Product      
Name: cystine/sulfocysteine:cation symporter
Synonym(s): TcyP, YdjN
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 48.662
Isoelectric point: 9.502
Motif(s):
 
Type Positions Sequence Comment
1 -> 21 MNFPLIANIVVFVVLLFALAQ UniProt: Helical.
29 -> 443 LAKKVLVGLVMGVVFGLALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLGKISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSDLSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAAIDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFAVHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFAASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGGATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQW
33 -> 53 VLVGLVMGVVFGLALHTIYGS UniProt: Helical.
73 -> 93 VQLLQMIVMPLVFASILSAVA UniProt: Helical.
106 -> 126 FLTIGTLLFTTLIAALVGVLV UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015293 - symporter activity
GO:0022857 - transmembrane transporter activity
GO:0000099 - sulfur amino acid transmembrane transporter activity
GO:0005416 - amino acid:cation symporter activity
GO:0015184 - L-cystine transmembrane transporter activity
biological_process GO:0006865 - amino acid transport
GO:0000101 - sulfur amino acid transport
GO:0006791 - sulfur utilization
GO:0055085 - transmembrane transport
GO:0003333 - amino acid transmembrane transport
GO:0015811 - L-cystine transport
Note(s): Note(s): ...[more].
Reference(s): [1] Berger P., et al., 2019
[2] Burguiere P., et al., 2004
[3] Gyaneshwar P., et al., 2005
[4] Zhou Y., et al., 2020
External database links:  
ALPHAFOLD:
 P77529
DIP:
 DIP-11776N
ECOCYC:
 G6934-MONOMER
ECOLIWIKI:
 b1729
INTERPRO:
 IPR001991
INTERPRO:
 IPR036458
PANTHER:
 PTHR42865
PFAM:
 PF00375
PRIDE:
 P77529
PRINTS:
 PR00173
PROSITE:
 PS00714
PROSITE:
 PS00713
REFSEQ:
 NP_416243
SMR:
 P77529
UNIPROT:
 P77529


Operon      
Name: tcyP         
Operon arrangement:
Transcription unit        Promoter
tcyP


Transcriptional Regulation      
Display Regulation             
Activated by: CysB


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2045 (cluster) 1811112 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Berger P., Kouzel IU., Berger M., Haarmann N., Dobrindt U., Koudelka GB., Mellmann A., 2019, Carriage of Shiga toxin phage profoundly affects Escherichia coli gene expression and carbon source utilization., BMC Genomics 20(1):504

 [2] Burguiere P., Auger S., Hullo MF., Danchin A., Martin-Verstraete I., 2004, Three different systems participate in L-cystine uptake in Bacillus subtilis., J Bacteriol 186(15):4875-84

 [3] Gyaneshwar P., Paliy O., McAuliffe J., Popham DL., Jordan MI., Kustu S., 2005, Sulfur and nitrogen limitation in Escherichia coli K-12: specific homeostatic responses., J Bacteriol 187(3):1074-90

 [4] Zhou Y., Imlay JA., 2020, Escherichia coli K-12 Lacks a High-Affinity Assimilatory Cysteine Importer., mBio 11(3)

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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