RegulonDB RegulonDB 11.1: Gene Form
   

astB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

astD astB astA astE spy astC BaeR BaeR CpxR astEp3 astEp3 TSS_2054 TSS_2054 spyp spyp TSS_2053 TSS_2053

Gene      
Name: astB    Texpresso search in the literature
Synonym(s): ECK1743, G6941, b1745, ydjT
Genome position(nucleotides): 1826916 <-- 1828259
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.58
External database links:  
ASAP:
 ABE-0005817
ECHOBASE:
 EB3752
ECOLIHUB:
 astB
OU-MICROARRAY:
 b1745
STRING:
 511145.b1745
COLOMBOS: astB


Product      
Name: N-succinylarginine dihydrolase
Synonym(s): AstB, YdjT
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 49.299
Isoelectric point: 6.059
Motif(s):
 
Type Positions Sequence Comment
3 -> 440 AWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQGLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSSASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFSVHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREASEAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLANLRARVNGFMAIEVPATQVSVSDTVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGYLNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFNALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQ
19 -> 28 AGLSFGNEAS UniProt: Substrate binding; Sequence Annotation Type: region of interest.
137 -> 138 HR UniProt: Substrate binding; Sequence Annotation Type: region of interest.
365 -> 365 C UniProt: Large decrease in activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016787 - hydrolase activity
GO:0009015 - N-succinylarginine dihydrolase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0006525 - arginine metabolic process
GO:0006527 - arginine catabolic process
GO:0019544 - arginine catabolic process to glutamate
GO:0019545 - arginine catabolic process to succinate
External database links:  
ALPHAFOLD:
 P76216
ECOCYC:
 SUCCARGDIHYDRO-MONOMER
ECOLIWIKI:
 b1745
INTERPRO:
 IPR037031
INTERPRO:
 IPR007079
PDB:
 1YNI
PDB:
 1YNF
PDB:
 1YNH
PFAM:
 PF04996
PRIDE:
 P76216
PRODB:
 PRO_000022176
REFSEQ:
 NP_416259
SMR:
 P76216
SWISSMODEL:
 P76216
UNIPROT:
 P76216


Operon      
Name: astCADBE         
Operon arrangement:
Transcription unit        Promoter
astCADBE
astCADBE
astCADBE


Transcriptional Regulation      
Display Regulation             
Activated by: NtrC, ArgR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2053 1825686 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2054 1825737 reverse nd [RS-EPT-CBR] [1]
  promoter astEp3 1827069 reverse nd [COMP-AINF] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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