RegulonDB RegulonDB 11.1: Gene Form
   

nimT gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nimT nimR yeaO yeaL NimR NimR NimR terminator anti-terminator anti-anti-terminator TSS_2124 TSS_2124 yeaOp3 yeaOp3 yeaOp4 yeaOp4 nimTp nimTp yeaMp1 yeaMp1 yeaNp9 yeaNp9 nimRp nimRp yeaMp6 yeaMp6 yeaNp4 yeaNp4

Gene      
Name: nimT    Texpresso search in the literature
Synonym(s): ECK1789, G6977, b1791, yeaN
Genome position(nucleotides): 1875673 --> 1876854
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.71
External database links:  
ASAP:
 ABE-0005962
ECHOBASE:
 EB3273
ECOLIHUB:
 yeaN
OU-MICROARRAY:
 b1791
STRING:
 511145.b1791
COLOMBOS: nimT


Product      
Name: 2-nitroimidazole exporter
Synonym(s): NimT, YeaN
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 41.208
Isoelectric point: 10.379
Motif(s):
 
Type Positions Sequence Comment
13 -> 33 IVLIAGILMIATTLRVTFTGA UniProt: Helical.
23 -> 349 ATTLRVTFTGAAPLLDTIRSAYSLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPYLLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLALNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTLFLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQRGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAAALSGMAQSVGYLLAA
53 -> 73 LLTTLPLLAFALISPLAAPVA UniProt: Helical.
81 -> 101 SLFAALLLICAGIAIRSLPSP UniProt: Helical.
102 -> 122 YLLFGGTAVIGGGIALGNVLL UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0022857 - transmembrane transporter activity
biological_process GO:0046677 - response to antibiotic
GO:0055085 - transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Jindal S., et al., 2019
External database links:  
ALPHAFOLD:
 P76242
ECOCYC:
 B1791-MONOMER
ECOLIWIKI:
 b1791
INTERPRO:
 IPR020846
INTERPRO:
 IPR011701
INTERPRO:
 IPR004747
INTERPRO:
 IPR036259
MODBASE:
 P76242
PFAM:
 PF07690
PRIDE:
 P76242
PROSITE:
 PS50850
REFSEQ:
 NP_416305
UNIPROT:
 P76242


Operon      
Name: nimT         
Operon arrangement:
Transcription unit        Promoter
nimT


Transcriptional Regulation      
Display Regulation             
Repressed by: NimR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yeaNp4 1875463 forward nd [COMP-AINF] [2]
  promoter yeaMp6 1875598 reverse nd [COMP-AINF] [2]
  promoter yeaMp1 1875648 reverse nd [COMP-AINF] [2]
  promoter yeaNp9 1875648 forward nd [COMP-AINF] [2]
  promoter yeaOp4 1876777 forward nd [COMP-AINF] [2]
  promoter yeaOp3 1876822 forward nd [COMP-AINF] [2]
  promoter TSS_2124 1876877 forward nd [RS-EPT-CBR] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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