RegulonDB RegulonDB 11.1: Gene Form
   

wcaM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

wcaM wcaL galF wcaK wcaMp1 wcaMp1 galFp galFp TSS_2398 TSS_2398 TSS_2397 TSS_2397 TSS_2396 TSS_2396

Gene      
Name: wcaM    Texpresso search in the literature
Synonym(s): ECK2037, G7094, b2043, yefK
Genome position(nucleotides): 2114502 <-- 2115896
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 47.1
External database links:  
ASAP:
 ABE-0006774
ECHOBASE:
 EB2521
ECOLIHUB:
 wcaM
OU-MICROARRAY:
 b2043
STRING:
 511145.b2043
COLOMBOS: wcaM


Product      
Name: putative colanic acid biosynthesis protein WcaM
Synonym(s): WcaM, YefK, orf1.3, orf20
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 51.316
Isoelectric point: 9.311
Motif(s):
 
Type Positions Sequence Comment
1 -> 28 MPFKKLSRRTFLTASSALAFLHTPFARA UniProt: Tat-type signal.
29 -> 464 LPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGEDGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLIIDNLTVSHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHFFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKINFRLPELRE UniProt: Colanic acid biosynthesis protein WcaM.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.2 - colanic acid (M antigen)
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
biological_process GO:0009103 - lipopolysaccharide biosynthetic process
GO:0045228 - slime layer polysaccharide biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Zhai Y., et al., 2002
External database links:  
ALPHAFOLD:
 P71244
ECOCYC:
 G7094-MONOMER
ECOLIWIKI:
 b2043
INTERPRO:
 IPR011050
INTERPRO:
 IPR023882
MODBASE:
 P71244
PRIDE:
 P71244
PRODB:
 PRO_000024217
REFSEQ:
 NP_416547
UNIPROT:
 P71244


Operon      
Name: wcaKLM         
Operon arrangement:
Transcription unit        Promoter
wcaKLM


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2396 2113809 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2397 2113829 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2398 2113953 reverse nd [RS-EPT-CBR] [2]
  promoter wcaMp1 2115934 reverse nd [COMP-AINF] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Zhai Y., Saier MH., 2002, The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes., Protein Sci 11(9):2196-207

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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