RegulonDB

Gene
Name:	arnD
Synonym(s):	ECK2249, G7169, b2256, pmrJ, yfbH
Genome position(nucleotides):	2370018 --> 2370908 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.86
External database links:

ASAP:	ABE-0007462
ECHOBASE:	EB3845
ECOLIHUB:	arnD
OU-MICROARRAY:	b2256
STRING:	511145.b2256
COLOMBOS:	arnD

Product

Name:

putative 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD

Synonym(s):

ArnD, PmrJ, YfbH

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

33.112

Isoelectric point:

7.644

Motif(s):

Type	Positions	Sequence
	2 -> 260	TKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDNMGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHEVGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIEAKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLLNRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFC
	15 -> 169	GTREGVPRLLEILSKHNIQASIFFSVGPDNMGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHEVGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIEAKEAFHLRY

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003824 - catalytic activity GO:0016787 - hydrolase activity GO:0016810 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds GO:0016811 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
	biological_process	GO:0005975 - carbohydrate metabolic process GO:0006629 - lipid metabolic process GO:0009103 - lipopolysaccharide biosynthetic process GO:0009245 - lipid A biosynthetic process GO:0046677 - response to antibiotic GO:0010041 - response to iron(III) ion GO:0036108 - 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process

Note(s):

Note(s): ...[more].

Evidence:

[AH] Author hypothesis

Reference(s):

[1] Breazeale SD., et al., 2005
[2] Gibbons HS., et al., 2005
[3] Groisman EA. 2001
[4] Nummila K., et al., 1995
[5] Trent MS., et al., 2001
[6] Zhou Z., et al., 1999

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

arnBCADTEF

Operon arrangement:

Transcription unit	Promoter
arnBCADTEF

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_2517	2367228		forward	nd	[RS-EPT-CBR]	[7]
	promoter	TSS_2518	2369128		forward	nd	[RS-EPT-CBR]	[7]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Breazeale SD., Ribeiro AA., McClerren AL., Raetz CR., 2005, A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose., J Biol Chem 280(14):14154-67
[2] Gibbons HS., Kalb SR., Cotter RJ., Raetz CR., 2005, Role of Mg2+ and pH in the modification of Salmonella lipid A after endocytosis by macrophage tumour cells., Mol Microbiol 55(2):425-40
[3] Groisman EA., 2001, The pleiotropic two-component regulatory system PhoP-PhoQ., J Bacteriol 183(6):1835-42
[4] Nummila K., Kilpelainen I., Zahringer U., Vaara M., Helander IM., 1995, Lipopolysaccharides of polymyxin B-resistant mutants of Escherichia coli are extensively substituted by 2-aminoethyl pyrophosphate and contain aminoarabinose in lipid A., Mol Microbiol 16(2):271-8
[5] Trent MS., Ribeiro AA., Doerrler WT., Lin S., Cotter RJ., Raetz CR., 2001, Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm., J Biol Chem 276(46):43132-44
[6] Zhou Z., Lin S., Cotter RJ., Raetz CR., 1999, Lipid A modifications characteristic of Salmonella typhimurium are induced by NH4VO3 in Escherichia coli K12. Detection of 4-amino-4-deoxy-L-arabinose, phosphoethanolamine and palmitate., J Biol Chem 274(26):18503-14
[7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.