RegulonDB RegulonDB 11.1: Gene Form
   

arnT gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

arnA arnT arnD arnF arnE pmrD TSS_2518 TSS_2518

Gene      
Name: arnT    Texpresso search in the literature
Synonym(s): ECK2250, G7170, b2257, pmrK, yfbI
Genome position(nucleotides): 2370908 --> 2372560
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.0
External database links:  
ASAP:
 ABE-0007464
ECHOBASE:
 EB3846
ECOLIHUB:
 arnT
OU-MICROARRAY:
 b2257
STRING:
 511145.b2257
COLOMBOS: arnT


Product      
Name: lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
Synonym(s): ArnT, PmrK, YfbI
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 62.543
Isoelectric point: 9.224
Motif(s):
 
Type Positions Sequence Comment
4 -> 237 VRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLASGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFTLRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKGKSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLTVLPWGLAIAQREPNFWHYF
7 -> 27 LIGLFAFIACYYLLPISTRLL UniProt: Helical.
81 -> 101 FGVRAGVIFATLLTAALVTWF UniProt: Helical.
110 -> 131 RLALLATVIYLSLFIVYAIGTY UniProt: Helical.
137 -> 154 FIAFWLVAGMCSFWLAMQ UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.3 - lipid A
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0103015 - 4-amino-4-deoxy-L-arabinose transferase activity
GO:0016757 - glycosyltransferase activity
GO:0016740 - transferase activity
GO:0016763 - pentosyltransferase activity
GO:0000030 - mannosyltransferase activity
biological_process GO:0006629 - lipid metabolic process
GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009245 - lipid A biosynthetic process
GO:0006493 - protein O-linked glycosylation
GO:0010041 - response to iron(III) ion
GO:0097502 - mannosylation
Note(s): Note(s): ...[more].
Reference(s): [1] Gibbons HS., et al., 2005
[2] Groisman EA. 2001
[3] Nummila K., et al., 1995
[4] Zhou Z., et al., 1999
External database links:  
ALPHAFOLD:
 P76473
CAZY:
 GT83
ECOCYC:
 G7170-MONOMER
ECOLIWIKI:
 b2257
INTERPRO:
 IPR022839
INTERPRO:
 IPR003342
PFAM:
 PF02366
PRIDE:
 P76473
PRODB:
 PRO_000022141
REFSEQ:
 NP_416760
UNIPROT:
 P76473


Operon      
Name: arnBCADTEF         
Operon arrangement:
Transcription unit        Promoter
arnBCADTEF


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2518 2369128 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Gibbons HS., Kalb SR., Cotter RJ., Raetz CR., 2005, Role of Mg2+ and pH in the modification of Salmonella lipid A after endocytosis by macrophage tumour cells., Mol Microbiol 55(2):425-40

 [2] Groisman EA., 2001, The pleiotropic two-component regulatory system PhoP-PhoQ., J Bacteriol 183(6):1835-42

 [3] Nummila K., Kilpelainen I., Zahringer U., Vaara M., Helander IM., 1995, Lipopolysaccharides of polymyxin B-resistant mutants of Escherichia coli are extensively substituted by 2-aminoethyl pyrophosphate and contain aminoarabinose in lipid A., Mol Microbiol 16(2):271-8

 [4] Zhou Z., Lin S., Cotter RJ., Raetz CR., 1999, Lipid A modifications characteristic of Salmonella typhimurium are induced by NH4VO3 in Escherichia coli K12. Detection of 4-amino-4-deoxy-L-arabinose, phosphoethanolamine and palmitate., J Biol Chem 274(26):18503-14

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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