RegulonDB RegulonDB 11.1: Gene Form
   

yfeW gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

murP yfeW yfeX TSS_2745 TSS_2745

Gene      
Name: yfeW    Texpresso search in the literature
Synonym(s): ECK2425, G7265, b2430
Genome position(nucleotides): 2548102 --> 2549406
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.88
External database links:  
ASAP:
 ABE-0008012
ECHOBASE:
 EB3916
ECOLIHUB:
 yfeW
OU-MICROARRAY:
 b2430
STRING:
 511145.b2430
COLOMBOS: yfeW


Product      
Name: penicillin binding protein 4B
Synonym(s): PBP4B, YfeW
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 47.752
Isoelectric point: 8.317
Motif(s):
 
Type Positions Sequence Comment
7 -> 25 YLSLLAVSCSVSAAKYPVL UniProt: Helical; Signal-anchor.
44 -> 412 DRWISQQVDVGYPSVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLASNTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGGFPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESVTGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTSTLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMFTTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMTIVMLSNKPHSPVADPQKNPNMFES

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.3 - proteins/peptides/glycopeptides
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.34 - murein turnover, recycling
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0031226 - intrinsic component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0008233 - peptidase activity
GO:0016787 - hydrolase activity
GO:0004180 - carboxypeptidase activity
GO:0009002 - serine-type D-Ala-D-Ala carboxypeptidase activity
GO:0008658 - penicillin binding
biological_process GO:0006508 - proteolysis
Note(s): Note(s): ...[more].
Evidence: [IDA-PURIFIED-PROTEIN-NH] Assay of protein purified to homogeneity from its native host
Reference(s): [1] Rao S., et al., 2020
[2] Vega D., et al., 2006
External database links:  
ALPHAFOLD:
 P77619
DIP:
 DIP-12021N
ECOCYC:
 G7265-MONOMER
ECOLIWIKI:
 b2430
INTERPRO:
 IPR012338
INTERPRO:
 IPR022849
INTERPRO:
 IPR001466
MODBASE:
 P77619
PFAM:
 PF00144
PRIDE:
 P77619
PROSITE:
 PS51257
REFSEQ:
 NP_416925
UNIPROT:
 P77619


Operon      
Name: murQP-yfeW         
Operon arrangement:
Transcription unit        Promoter
murQP
murQP
murQP
murQP-yfeW


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2745 2549935 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3

 [2] Vega D., Ayala JA., 2006, The DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli., Arch Microbiol 185(1):23-7

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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