RegulonDB

Gene
Name:	mlaB
Synonym(s):	ECK3180, G7658, b3191, yrbB
Genome position(nucleotides):	3336963 <-- 3337256 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.4
External database links:

ASAP:	ABE-0010487
ECHOBASE:	EB2649
ECOLIHUB:	mlaB
OU-MICROARRAY:	b3191
STRING:	511145.b3191
COLOMBOS:	mlaB

Product

Name:

intermembrane phospholipid transport system protein MlaB

Synonym(s):

MlaB, YrbB

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

10.68

Isoelectric point:

4.52

Motif(s):

Type	Positions	Sequence
	14 -> 90	LALSGELDQDVLLPLWEMREEAVKGITCIDLSRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNL
	52 -> 52	T
	1 -> 97	MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDLSRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR

Classification:

Multifun Terms (GenProtEC)
		4 - transport
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0032991 - protein-containing complex
	molecular_function	GO:0120014 - phospholipid transfer activity GO:0005515 - protein binding GO:0016887 - ATPase activity
	biological_process	GO:0120010 - intermembrane phospholipid transfer GO:0006974 - cellular response to DNA damage stimulus GO:0046677 - response to antibiotic GO:0015914 - phospholipid transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Ekiert DC., et al., 2017
[2] Hughes GW., et al., 2019
[3] Kolich LR., et al., 2020

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

MODBASE:

PDB:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

mlaFEDCB

Operon arrangement:

Transcription unit	Promoter
mlaFEDCB	mlaFp3
mlaFEDCB	mlaFp2
mlaFEDCB	mlaFp1

Transcriptional Regulation
	Display Regulation
Activated by:	MarA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_3501 (cluster)	3336555	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3336554 to 3336555, where the highest frequency is at position 3334577. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[4]
promoter	ibaGp9	3336954	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 5.058. P-value: 1.864e-5 Read more >	[ICWHO]	[5]
promoter	ibaGp4	3336983	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 2.91. P-value: 6.573e-5 Read more >	[ICWHO]	[5]
promoter	TSS_3502	3337936	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_3503	3338185	reverse	nd	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Ekiert DC., Bhabha G., Isom GL., Greenan G., Ovchinnikov S., Henderson IR., Cox JS., Vale RD., 2017, Architectures of Lipid Transport Systems for the Bacterial Outer Membrane., Cell 169(2):273-285.e17
[2] Hughes GW., Hall SCL., Laxton CS., Sridhar P., Mahadi AH., Hatton C., Piggot TJ., Wotherspoon PJ., Leney AC., Ward DG., Jamshad M., Spana V., Cadby IT., Harding C., Isom GL., Bryant JA., Parr RJ., Yakub Y., Jeeves M., Huber D., Henderson IR., Clifton LA., Lovering AL., Knowles TJ., 2019, Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system., Nat Microbiol 4(10):1692-1705
[3] Kolich LR., Chang YT., Coudray N., Giacometti SI., MacRae MR., Isom GL., Teran EM., Bhabha G., Ekiert DC., 2020, Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation., Elife 9
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78