RegulonDB

Gene
Name:	bsmA
Synonym(s):	ECK4185, G7852, b4189, yjfO
Genome position(nucleotides):	4416441 <-- 4416770 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	48.79
External database links:

ASAP:	ABE-0013706
ECHOBASE:	EB2382
ECOLIHUB:	bsmA
OU-MICROARRAY:	b4189
STRING:	511145.b4189
COLOMBOS:	bsmA

Product

Name:

DUF1471 domain-containing putative lipoprotein BsmA

Synonym(s):

BsmA, YjfO

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane,periplasmic space

Molecular weight:

12.038

Isoelectric point:

9.794

Motif(s):

Type	Positions	Sequence
	1 -> 24	MVSRKRNSVIYRFASLLLVLMLSA
	53 -> 108	LQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYSQAILYR
	25 -> 109	CSALQGTPQPAPPVTDHPQEIRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYSQAILYRK

Classification:

Multifun Terms (GenProtEC)
		5 - cell processes --> 5.5 - adaptations --> 5.5.4 - pH
		5 - cell processes --> 5.5 - adaptations --> 5.5.6 - other (mechanical, nutritional, oxidative stress)
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0030288 - outer membrane-bounded periplasmic space GO:0005886 - plasma membrane
	biological_process	GO:0006974 - cellular response to DNA damage stimulus GO:0044010 - single-species biofilm formation

Note(s):

Note(s): ...[more].

Evidence:

[IEP] Inferred from expression pattern
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Rao S., et al., 2020
[2] Weber MM., et al., 2010

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

bsmA

Operon arrangement:

Transcription unit	Promoter
bsmA	bsmAp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yjfPp4	4416756	forward	nd	[ICWHO]	[3]
promoter	yjfPp5	4416935	forward	nd	[ICWHO]	[3]
promoter	TSS_5054	4416936	forward	nd	[RS-EPT-CBR]	[4]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3
[2] Weber MM., French CL., Barnes MB., Siegele DA., McLean RJ., 2010, A previously uncharacterized gene, yjfO (bsmA), influences Escherichia coli biofilm formation and stress response., Microbiology 156(Pt 1):139-47
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.