RegulonDB RegulonDB 11.1: Gene Form
   

xapA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

xapB xapA yfeN XapR XapR terminator xapAp2 xapAp2 xapAp xapAp xapBp3 xapBp3 xapBp2 xapBp2 xapBp1 xapBp1

Gene      
Name: xapA    Texpresso search in the literature
Synonym(s): ECK2401, G85, b2407, pndA
Genome position(nucleotides): 2524045 <-- 2524878
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.68
External database links:  
ASAP:
 ABE-0007927
CGSC:
 9
ECHOBASE:
 EB4152
ECOLIHUB:
 xapA
MIM:
 613179
NCBI-GENE:
 946878
OU-MICROARRAY:
 b2407
STRING:
 511145.b2407
COLOMBOS: xapA


Shine dalgarno      
Sequence: ccctacagaaAAAGGAtatgTAT


Product      
Name: xanthosine phosphorylase
Synonym(s): PndA, XapA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 29.835
Isoelectric point: 6.504
Motif(s):
 
Type Positions Sequence Comment
27 -> 273 RVAFILGSGLGALADQIENAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIRTFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSLANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFL
85 -> 87 RGH UniProt: Phosphate binding; Sequence Annotation Type: region of interest.
144 -> 144 P UniProt: In Ref. 1; CAA52049..
191 -> 191 Y UniProt: No detectable activity with xanthosine as substrate, but largely retains its activity against other substrates, namely inosine and guanosine, although with altered affinities, higher and lower respectively, and clearly reduced maximal velocities for both..
239 -> 239 N UniProt: Catalyzes the phosphorolysis of adenosine with moderate efficiency, and essentially has lost all activity against the 6-oxo-purine substrates xanthosine, inosine and guanosine..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016757 - glycosyltransferase activity
GO:0016740 - transferase activity
GO:0004731 - purine-nucleoside phosphorylase activity
GO:0016763 - pentosyltransferase activity
GO:0047724 - inosine nucleosidase activity
GO:0042802 - identical protein binding
GO:0047975 - guanosine phosphorylase activity
biological_process GO:0006139 - nucleobase-containing compound metabolic process
GO:0009116 - nucleoside metabolic process
GO:0015949 - nucleobase-containing small molecule interconversion
GO:0006152 - purine nucleoside catabolic process
GO:0055086 - nucleobase-containing small molecule metabolic process
GO:0034355 - NAD salvage
GO:0006148 - inosine catabolic process
GO:0006149 - deoxyinosine catabolic process
GO:0006161 - deoxyguanosine catabolic process
GO:0034214 - protein hexamerization
GO:0046115 - guanosine catabolic process
GO:1903228 - xanthosine catabolic process
External database links:  
ALPHAFOLD:
 P45563
ECOCYC:
 XANTHOSINEPHOSPHORY-MONOMER
ECOLIWIKI:
 b2407
INTERPRO:
 IPR018099
INTERPRO:
 IPR035994
INTERPRO:
 IPR011268
INTERPRO:
 IPR010943
INTERPRO:
 IPR000845
MODBASE:
 P45563
PANTHER:
 PTHR11904
PDB:
 1YQQ
PDB:
 1YQU
PDB:
 1YR3
PFAM:
 PF01048
PRIDE:
 P45563
PRODB:
 PRO_000024228
PROSITE:
 PS01240
REFSEQ:
 NP_416902
SMR:
 P45563
UNIPROT:
 P45563


Operon      
Name: xapAB         
Operon arrangement:
Transcription unit        Promoter
xapB
xapB
xapB
xapAB
xapAB


Transcriptional Regulation      
Display Regulation             
Activated by: XapR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References



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