RegulonDB

RegulonDB RegulonDB 10.9: Gene Form

insJK gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	insJK
Synonym(s):	G0-10624
Genome position(nucleotides):	3720680 --> 3722049 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	44.09
External database links:

ECOLIHUB:	insJK
COLOMBOS:	insJK

Product

Name:

IS150 protein InsAB

Synonym(s):

InsJK

Sequence:

Get amino acid sequence Fasta Format

Molecular weight:

53.021

Isoelectric point:

10.204

Motif(s):

Type	Positions	Sequence
	397 -> 451	ECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQT
	68 -> 114	RIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLKVYEERGEAGLRA
	216 -> 269	VKKRISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKR
	292 -> 390	TRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNC
	10 -> 59	KRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPK

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
	molecular_function	GO:0004803 - transposase activity
	biological_process	GO:0006313 - transposition, DNA-mediated

Note(s):

Note(s): ...[more].

Evidence:

[IMP] Inferred from mutant phenotype

Reference(s):

[1] Haas M., et al., 2002

External database links:

ECOCYC:

PFAM:

PFAM:

PFAM:

PFAM:

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	insJp4	3720594	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 2.696. P-value: 6.841e-5 Read more >	[ICWHO]	[2]
promoter	hokAp6	3720810	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 4.275. P-value: 4.362e-5 Read more >	[ICWHO]	[2]
promoter	TSS_4162	3721864	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_4163	3722550	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_4164 (cluster)	3723367	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3723366 to 3723367, where the highest frequency is at position 3721389. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]
promoter	TSS_4165	3723961	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_4166 (cluster)	3724383	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3724382 to 3724383, where the highest frequency is at position 3722406. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Haas M., Rak B., 2002, Escherichia coli insertion sequence IS150: transposition via circular and linear intermediates., J Bacteriol 184(21):5833-41
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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