RegulonDB

Gene
Name:	micL
Synonym(s):	ECK4574, G0-16601, b4717, ryeF, slrA
Genome position(nucleotides):	1958441 <-- 1958520 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	46.25
Reference(s):	[1] Klein G., et al., 2014

Product

Name:

MicL

Synonym(s):

MicL-S, RyeF, SlrA, ig:1179, ryeF, sirA, small regulatory RNA MicL-S

Type:

small RNA

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
	molecular_function	GO:0005515 - protein binding GO:0003729 - mRNA binding
	biological_process	GO:0040033 - negative regulation of translation, ncRNA-mediated GO:1902201 - negative regulation of bacterial-type flagellum-dependent cell motility

Note(s):

Note(s): ...[more].

Evidence:

[IE] Inferred from experiment
[IGI] Inferred from genetic interaction
[IMP] Inferred from mutant phenotype

Reference(s):

[2] Guo MS., et al., 2014
[1] Klein G., et al., 2014
[3] Nicoloff H., et al., 2017
[4] Zhang A., et al., 2003

External database links:

ECOCYC:

RNA0-386

Operon

Name:

micL

Operon arrangement:

Transcription unit	Promoter
micL	micLp
micL	micLp2

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_2221	1958648		reverse	nd	[RS-EPT-CBR]	[5]
	promoter	cutCp2	1959267		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.288. P-value: 9.611e-5 Read more >	[ICWHO]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Klein G., Kobylak N., Lindner B., Stupak A., Raina S., 2014, Assembly of lipopolysaccharide in Escherichia coli requires the essential LapB heat shock protein., J Biol Chem 289(21):14829-53
[2] Guo MS., Updegrove TB., Gogol EB., Shabalina SA., Gross CA., Storz G., 2014, MicL, a new σE-dependent sRNA, combats envelope stress by repressing synthesis of Lpp, the major outer membrane lipoprotein., Genes Dev 28(14):1620-34
[3] Nicoloff H., Gopalkrishnan S., Ades SE., 2017, Appropriate Regulation of the σ^E-Dependent Envelope Stress Response Is Necessary To Maintain Cell Envelope Integrity and Stationary-Phase Survival in Escherichia coli, J Bacteriol 199(12)
[4] Zhang A., Wassarman KM., Rosenow C., Tjaden BC., Storz G., Gottesman S., 2003, Global analysis of small RNA and mRNA targets of Hfq., Mol Microbiol 50(4):1111-24
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78