RegulonDB

Gene
Name:	chbF
Synonym(s):	ECK1732, EG10144, b1734, celF, ydjD
Genome position(nucleotides):	1817148 <-- 1818500
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	46.78
External database links:

ASAP:	ABE-0005785
CGSC:	17734
ECHOBASE:	EB0142
ECOLIHUB:	chbF
OU-MICROARRAY:	b1734
STRING:	511145.b1734
COLOMBOS:	chbF

Product

Name:

monoacetylchitobiose-6-phosphate hydrolase

Synonym(s):

CelF, ChbF, YdjD

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

50.512

Isoelectric point:

6.326

Motif(s):

Type	Positions	Sequence	Comment
	2 -> 450	SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKPKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLINGKSRFAELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGQIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH	UniProt: 6-phospho-beta-glucosidase.
	5 -> 73	LKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKPKLDIIFDLCQRMIDNAGVPMKLYKTLD	UniProt: NAD.
	6 -> 187	KVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKPKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKMFIRDVLM
	199 -> 415	FGLNHMVFIKDVLINGKSRFAELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGQIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLV

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0103047 - methyl beta-D-glucoside 6-phosphate glucohydrolase activity GO:0003824 - catalytic activity GO:0016787 - hydrolase activity GO:0046872 - metal ion binding GO:0004553 - hydrolase activity, hydrolyzing O-glycosyl compounds GO:0016798 - hydrolase activity, acting on glycosyl bonds GO:0016616 - oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0008706 - 6-phospho-beta-glucosidase activity GO:0042802 - identical protein binding
	biological_process	GO:0008152 - metabolic process GO:0005975 - carbohydrate metabolic process GO:0052777 - diacetylchitobiose catabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Guzzo A., et al., 1994
[2] Parker LL., et al., 1990
[3] Plumbridge J., et al., 2004

External database links:

ALPHAFOLD:

CAZY:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, ChbR
Repressed by:	NagC, ChbR

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	chbGp4	1817242		reverse	nd	[COMP-AINF]	[4]
	promoter	chbGp5	1817312		reverse	nd	[COMP-AINF]	[4]

Reference(s)
[1] Guzzo A., DuBow MS., 1994, A luxAB transcriptional fusion to the cryptic celF gene of Escherichia coli displays increased luminescence in the presence of nickel., Mol Gen Genet 242(4):455-60
[2] Parker LL., Hall BG., 1990, Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12., Genetics 124(3):455-71
[3] Plumbridge J., Pellegrini O., 2004, Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP., Mol Microbiol 52(2):437-49
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78