RegulonDB RegulonDB 11.1: Gene Form
   

dgkA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

plsB lexA dgkA LexA LexA BasR anti-terminator terminator TSS_4882 TSS_4882 lexAp lexAp dgkAp2 dgkAp2 plsBp1 plsBp1 plsBp2 plsBp2 dgkAp1 dgkAp1 TSS_4879 TSS_4879 TSS_4878 TSS_4878 TSS_4877 TSS_4877

Gene      
Name: dgkA    Texpresso search in the literature
Synonym(s): ECK4034, EG10224, b4042
Genome position(nucleotides): 4256637 --> 4257005
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.76
External database links:  
ASAP:
 ABE-0013235
CGSC:
 862
ECHOBASE:
 EB0220
ECOLIHUB:
 dgkA
OU-MICROARRAY:
 b4042
STRING:
 511145.b4042
COLOMBOS: dgkA


Product      
Name: diacylglycerol kinase
Synonym(s): DgkA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 13.245
Isoelectric point: 6.504
Motif(s):
 
Type Positions Sequence Comment
2 -> 122 ANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSHFG UniProt: Diacylglycerol kinase.
14 -> 19 AAGYSW UniProt: Substrate binding; Sequence Annotation Type: region of interest.
15 -> 15 A UniProt: 49% of wild-type specific activity. Inactivates wild-type subunits; when associated with S-73..
16 -> 117 GYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILL
19 -> 19 W UniProt: 60% of wild-type activity. 41% of wild-type activity; when associated with L-26..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.4 - fatty acids and phosphatidic acid
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.1 - phospholipid
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004143 - diacylglycerol kinase activity
GO:0042802 - identical protein binding
biological_process GO:0006629 - lipid metabolic process
GO:0006654 - phosphatidic acid biosynthetic process
GO:0008610 - lipid biosynthetic process
GO:0008654 - phospholipid biosynthetic process
GO:0016310 - phosphorylation
GO:0009411 - response to UV
External database links:  
ALPHAFOLD:
 P0ABN1
DIP:
 DIP-60228N
ECOCYC:
 DIACYLGLYKIN-MONOMER
ECOLIWIKI:
 b4042
INTERPRO:
 IPR000829
INTERPRO:
 IPR033718
INTERPRO:
 IPR036945
PANTHER:
 PTHR34299
PDB:
 4D2E
PDB:
 4UP6
PDB:
 4UXW
PDB:
 4UXX
PDB:
 4UXZ
PDB:
 4UYO
PDB:
 5D56
PDB:
 5D57
PDB:
 5D6I
PDB:
 5DWK
PDB:
 4CK0
PDB:
 4CJZ
PDB:
 4BRR
PDB:
 4BRB
PDB:
 4BPD
PDB:
 3ZE5
PDB:
 3ZE4
PDB:
 2KDC
PDB:
 3ZE3
PFAM:
 PF01219
PRIDE:
 P0ABN1
PRODB:
 PRO_000022432
PROSITE:
 PS01069
REFSEQ:
 NP_418466
SMR:
 P0ABN1
UNIPROT:
 P0ABN1


Operon      
Name: dgkA         
Operon arrangement:
Transcription unit        Promoter
dgkA
dgkA


Transcriptional Regulation      
Display Regulation             
Activated by: BasR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4877 4255382 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4878 4255992 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4879 4256011 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4882 4257204 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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