RegulonDB

Gene
Name:	hypA
Synonym(s):	ECK2721, EG10483, b2726
Genome position(nucleotides):	2850647 --> 2850997
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.99
External database links:

ASAP:	ABE-0008958
CGSC:	33104
ECHOBASE:	EB0478
ECOLIHUB:	hypA
OU-MICROARRAY:	b2726
STRING:	511145.b2726
COLOMBOS:	hypA

Shine dalgarno
Sequence:	atagttaatgGGAGGCgatATG

Product

Name:

hydrogenase 3 nickel incorporation protein HypA

Synonym(s):

HypA, accessory protein for nickel incorporation into hydrogenase 3

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane,cytosol

Molecular weight:

13.168

Isoelectric point:

5.181

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 113	MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSLAFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVADDGLQIRRIEI
	2 -> 2	H	UniProt: Does not affect interaction with HycE..

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
Gene Ontology Terms (GO)
	cellular_component	GO:1905360 - GTPase complex GO:1902695 - metallochaperone complex GO:0005829 - cytosol GO:0016020 - membrane GO:0005886 - plasma membrane GO:0031234 - extrinsic component of cytoplasmic side of plasma membrane
	molecular_function	GO:0005515 - protein binding GO:0046872 - metal ion binding GO:0016151 - nickel cation binding GO:0008270 - zinc ion binding GO:0042802 - identical protein binding GO:0016530 - metallochaperone activity
	biological_process	GO:0110147 - protein maturation by nickel ion transfer GO:0006464 - cellular protein modification process GO:0051604 - protein maturation

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Jacobi A., et al., 1992

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

hypABCDE-fhlA

Operon arrangement:

Transcription unit	Promoter
hypABCDE	hypAp
hypABCDE-fhlA	hypAp
hypBCDE	hypBp
hypBCDE-fhlA	hypBp

Transcriptional Regulation
	Display Regulation
Activated by:	FhlA, IHF
Repressed by:	NsrR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Jacobi A., Rossmann R., Bock A., 1992, The hyp operon gene products are required for the maturation of catalytically active hydrogenase isoenzymes in Escherichia coli., Arch Microbiol 158(6):444-51