RegulonDB RegulonDB 11.1: Gene Form
   

hypB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hypD hypB hypA hypC hypE FNR FNR ModE hypBp hypBp

Gene      
Name: hypB    Texpresso search in the literature
Synonym(s): ECK2722, EG10484, b2727, hydB
Genome position(nucleotides): 2851001 --> 2851873
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.21
External database links:  
ASAP:
 ABE-0008960
CGSC:
 33110
ECHOBASE:
 EB0479
ECOLIHUB:
 hypB
OU-MICROARRAY:
 b2727
STRING:
 511145.b2727
COLOMBOS: hypB


Shine dalgarno      
Sequence: gaaatagaccAGGAGTgagcgATG


Product      
Name: hydrogenase isoenzymes nickel incorporation protein HypB
Synonym(s): HydB, HypB, accessory protein for nickel incorporation into hydrogenase isoenzymes
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 31.565
Isoelectric point: 5.397
Motif(s):
 
Type Positions Sequence Comment
2 -> 2 C UniProt: Decreases nickel content. Disruption of the N-terminal metal-binding site; when associated with A-5 and A-7..
5 -> 5 C UniProt: Decreases nickel content. Disruption of the N-terminal metal-binding site; when associated with A-3 and A-7..
7 -> 7 C UniProt: Decreases nickel content. Decreases hydrogenase activity of the cell. Disruption of the N-terminal metal-binding site; when associated with A-3 and A-5..
36 -> 36 P UniProt: In Ref. 1; CAA38413..
78 -> 80 LEV UniProt: Disrupts interaction with HypA. Can only partially restore hydrogenase production in a hypB deletion mutant..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
  2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
Gene Ontology Terms (GO)  
cellular_component GO:1905360 - GTPase complex
GO:1902695 - metallochaperone complex
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0046872 - metal ion binding
GO:0000166 - nucleotide binding
GO:0003924 - GTPase activity
GO:0016151 - nickel cation binding
GO:0005525 - GTP binding
GO:0008270 - zinc ion binding
GO:0042803 - protein homodimerization activity
GO:0016530 - metallochaperone activity
GO:0097216 - guanosine tetraphosphate binding
biological_process GO:0110147 - protein maturation by nickel ion transfer
GO:0051604 - protein maturation
Note(s): Note(s): ...[more].
Reference(s): [1] Edmonds KA., et al., 2021
External database links:  
ALPHAFOLD:
 P0AAN3
DIP:
 DIP-36428N
ECOCYC:
 EG10484-MONOMER
ECOLIWIKI:
 b2727
INTERPRO:
 IPR004392
INTERPRO:
 IPR003495
INTERPRO:
 IPR027417
MINT:
 P0AAN3
MODBASE:
 P0AAN3
PANTHER:
 PTHR30134
PFAM:
 PF02492
PRIDE:
 P0AAN3
PRODB:
 PRO_000022973
REFSEQ:
 NP_417207
SMR:
 P0AAN3
UNIPROT:
 P0AAN3


Operon      
Name: hypABCDE-fhlA         
Operon arrangement:
Transcription unit        Promoter
hypABCDE
hypABCDE-fhlA
hypBCDE
hypBCDE-fhlA


Transcriptional Regulation      
Display Regulation             
Activated by: FhlA, FNR, IHF
Repressed by: NsrR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Edmonds KA., Jordan MR., Giedroc DP., 2021, COG0523 proteins: a functionally diverse family of transition metal-regulated G3E P-loop GTP hydrolases from bacteria to man., Metallomics 13(8)

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